乙肝患者HBsAg定量与HBV-DNA及乙肝标志物模式的比较分析

Relationship between quantitative HBsAg by chemiluminescent immunoassay,HBV-DNA and HBV-M in patients with hepatitis B

目的探讨化学发光化法乙肝表面抗原定量测定与HBV-DNA水平及乙肝病毒标志物模式之间的相互关系。方法运用化学发光法、荧光定量PCR(FQ-PCR)及ELISA三种方法同时检测258份血清标本,并对其结果应用统计学方法加以对比分析。结果258份乙肝患者血清HBsAg含量与HBV-DNA及乙肝病毒标志物检测,显示其具有良好的相关性。结论化学发光法定量检测HBsAg能较准确、快速地反映体内乙肝病毒复制情况。

Objective To explore the relationship between quantitative HBsAg by chemiluminescent immunoassay （CMIA）, hepatitis B virus （HBV） DNA level and HBV marker （HBV-M）. Methods In sera of 258 patients, HBsAg was detected by CMIA, and HBV-DNA was detected by florescence quantitative PCR （FQ-PCR）, while HBV-M was detected by enzyme linked immuno sorbent assay （ELISA）. Results There were well correlation between HBsAg, HBV-DNA and HBVM in sera of 258 patients. Conclusions The level of HBsAg detected by CMIA can better reflect the replication of HBV.