期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

MK-801对吗啡戒断大鼠中枢NMDA_(1A)受体mRNA表达的影响 被引量：3

Effects of MK-801 on NMDA_(1A) receptor mRNA of central nerves system in morphine withdrawal rats
原文传递
导出
摘要 Objective To observe the effect of MK-801（non-competitive NMDA receptor antagonist） on NMDA1A RmRNA of the spinal cord and brainstem in morphine withdrawal rats. Methods Thirty-two rats were divided into four groups （n=8）: control group, dependence group, withdrawal group and MK-801 group. The rats were killed 1 hour after naloxone challenge. The expression of NMDA 1A RmRNA was determined by methods of RT-PCR. Results The expression of NMDA 1A RmRNA in the spinal cord （0.84±0.16） and brainstem （0.74±0.14） significantly increased in morphine dependent group than in the control group （0.65±0.15） and （0.55±0.12） （P<0.05） and increased to a great higher extent in morphine withdrawal group （1.09±0.20） and （0.98±0.17） （P< 0.05 ）. The expression of NMDA1A RmRNA in the spinal cord （0.78±0.18） and brainstem （0.69±0.14） of MK-801 group was lower than that of morphine withdrawal group （P<0.01）.Conclusion MK-801 significantly inhibits the expression of NMDA1A RmRNA of central nerves system in morphine withdrawal rats. Objective To observe the effect of MK-801 （non-competitive NMDA receptor antagonist） on NMDAIA RmRNA of the spinal cord and brainstem in morphine withdrawal rats. Methods Thirty-two rats were divided into four groups （ n = 8） ： control group, dependence group, withdrawal group and MK-801 group. The rats were killed 1 hour after naloxone challenge. The expression of NM- DAIA RmRNA was determined by methods of RT-PCR. Results The expression of NMDA1A RmRNA in the spinal cord （0.84±0.16） and brainstem （0.74± 0.14） significantly increased in morphine dependent group than in the control group （0.65±0.15 ） and （0.55±0.12） （ P 〈 0.05 ） and increased to a great higher extent in morphine withdrawal group （ 1.09±0.20） and （0.98±0.17 ） （ P 〈 0.05 ）. The expression of NMDA1A RmRNA in the spinal cord （0.78±0.18） and brainstem （0.69±0.14） of MK-801 group was lower than that of morphine withdrawal group （P 〈 0.01 ）. Conclusion MK-801 significantly inhibits the expression of NMDA1A RmRNA of central nerves system in morphine withdrawal rats.
作者 席宏杰 刘晶 岳子勇 李文志 朱大岭
机构地区 哈尔滨医科大学附属第二医院麻醉科
出处 《国际麻醉学与复苏杂志》 CAS 2006年第4期218-220,共3页 International Journal of Anesthesiology and Resuscitation
基金 黑龙江省自然基金资助项目(项目编号:D0202) 博士后基金资助项目
关键词 非竞争性NMDA受体拮抗剂 MK-801 吗啡戒断大鼠 mRNA表达 中枢 吗啡耐受 研究发现 阿片类药物 免疫组化法 A受体表达 MK-801 Morphine Withdrawal NMDA receptor
分类号 R749.64 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献10

  • 1Trujillo KA,Akil H.Inhibition of morphine tolerance and dependence by the NMDA receptor antagonist MK-801.Science,1991,251(4989):85-87.
  • 2徐振波,吴家文,汪秉康,王文东.吗啡染毒大鼠死后体内吗啡的分布研究[J].法医学杂志,1997,13(2):71-74. 被引量：11
  • 3吴宁,李锦.阿片依赖病理过程中NMDA受体与阿片受体的相互作用及分子机制[J].中国药理学通报,2004,20(1):1-4. 被引量：8
  • 4王玢,罗非,韩济生.参与阿片身体依赖的核团及受体后过程[J].中国神经科学杂志,1999,15(1):63-68. 被引量：10
  • 5Koyuncuoglu H,Nurten AY,Amanturk P,et al.The importance of the number of NMDA receptors in the development of supersensitivity or tolerance to and dependence on morphine.Pharmacol Res,1999,39(4):311-319.
  • 6郭瑞鲜,冯鉴强,陈培熹,王竞菲.脊髓NMDAR1受体在吗啡耐受过程中的变化[J].解剖学研究,2000,22(2):120-122. 被引量：6
  • 7Brenner GJ,Ji RR,Shaffer S,et al.Peripheral noxious stimulation induces phosphorylation of the NMDA receptor NR1 subunit at the PKC-dependent site,serine-896,in spinal cord dorsal horn neurons.Neu-rosci,2004,20(2):375-384.
  • 8Fang L,Wu J,Lin Q,et al.Protein kinases regulate the phosphorylation of the GluR1 subunit of AMPA receptors of spinal cord in rats following noxious stimulation.Mol Brain Res,2003,118(1-2):160-165.
  • 9Raval AP,Dave KR,Mochly-Rosen D,et al.Epsilon PKC is required for the induction of tolerance by ischemic and NMDA-mediated preconditioning in the organotypic hippocampal splice.J Neurosci,2003,23(2):384-391.
  • 10Yashpal K,Fisher K,Chabot JG,et al.Differential effects of NMDA and group ⅠmGluR antagonists on both nociception and spinal cord protein kinase C translocation in the formalin test and a model of neuropathic pain in rats.Pain,2001,94(1):17-29.

二级参考文献12

  • 1Trujillo KA,Akil H. Inhibition ofmorphine tolerance and dependence by the NMDA receptor antagonist MK-801.Science,1991,251:85～87.
  • 2Ben Eliyahu S,Marek P,Vaccarino AL,et al. The NMDA receptor antagonist MK-801prevent long-lasting non-associative morphine tolerance in the rat. Brain Res,1992,575:304～308.
  • 3Koyuncuoglu H,Nurten A,Yamanturk P,et al. The importance of the number of NMDAreceptors in the development of supersensitivity or tolerance to and dependence onmorphine. Pharmacol. Res,1999,39:311～319.
  • 4Mao J,price DD,Mayer DJ. Mechanisms of hyperalgesia and morphine tolerance:acurrent view of their possible interactions. Pain,1995,62:259～274.
  • 5Dunbar S,Yaksh TL. Concurrent spinal infusion of MK801 blocks spinal tolerance anddependence induced by chronic intrathecal morphine in the rat. Anesthesiology,1996,84:1177～1188.
  • 6Mao J,Price DD,Phillips LL,et al. Increses in protein kinase C gammaimmunoreactivity in the spinal cord of rats associated with tolerance to the analgesiceffects of morphine. Brain Res,1995,677:257～267.
  • 7Mao J,Price DD,Mayer DJ. Thermal hyperalgesia in association with the developmentof morphine tolerance in rats Roles of excitatory amino acid receptors and protein kinaseC. J. Neurosci,1994,14:2301～2312.
  • 8Buccafusco JJ,Terry AV,Shuster JL. Spinal NMDA receptor-nitric oxide mediation ofthe expression of morphine withdrawal symptoms in the rat. Brain Res,1995,679:189～199.
  • 9Chen L and Huang LYM,Sustained potentiation of NMDA receptor mediated glutamateresponses through activation of protein kinase C by μ opioid,Neuron,1991,7:319～326.
  • 10.Chen L,Huang LYM. Protein kinase C reduces Mg2+ block of NMDA receptor channelsas a mechanism of modulation. Nature. 1992,356:521～523.

共引文献30

同被引文献43

引证文献3

二级引证文献22

国际麻醉学与复苏杂志
2006年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部