川麦冬脱病毒和组织培养技术的研究

Studies on Viru-free Culture and Chromosome Identification Technique of Ophiopogon Japonicus(L.f.)Ker-Gawl

采用茎尖分生组织培养技术,获得了川麦冬的脱病毒试管苗。通过川麦冬组织培养技术的正交试验及优化筛选,筛选出最佳的培养基组成,用于脱病毒苗的快速繁殖。建立了川麦冬根尖染色体鉴定的最佳条件。结果表明,川麦冬最适繁殖培养基MS+BA(6-卞基腺嘌呤)2.0 mg/L+NAA(萘乙酸)0.5 mg/L;川麦冬最佳诱导愈伤培养基:MS+BA1.5 mg/L+IAA(吲哚乙酸)0.1 mg/L+2,4-D(2,4-二氯苯氧乙酸)1.0 mg/L;通过在培养基中添加不同浓度生长素,得到适合川麦冬的生根培养基:1/2MS+IAA 0.5 mg/L+ABT 0.5 mg/L。染色体鉴定结果表明:川麦冬的染色体为2n=68。

Virus-free materials of Ophiopogon japonicus were obtained from meristem culture in vitro. A series of optimization experiments for the composition of cultural medium and concentration of phytohormones were investigated with a view to accelerate the propagation of virus-free materials. The most suitable media of Ophiopogon japonicus for rapid-propagation was established and optimized by orthogohal test in tissue culture process. The obtained results indicated that the most suitable rapid-propagation media of Ophiopogon japonicus is MS＋BA2. 0 mg/L ＋NAA0. 5 mg/L. The best inducing medium for callus is MS＋BA1.5 mg/L＋IAA0. 1 mg/L＋2,4-D 1.0 mg/L. and its rooting media is 1/2MS＋IAA 0. 5 mg/L＋ABT 0. 5 mg/L. The best condition of chromosome deter mination was established. The chromosome number of Ophiopogon japonicus was 2n= 68. The above research laid the foundation for preserving the plant resource and breeding excellent lines of Ophiopogon japonicus.