摘要
在国内首次采用聚合酶链反应扩增新生隐球菌 rDNA 部分特异性序列的方法诊断隐球菌性脑膜炎。所试11株新生隐球菌均扩增产生预期长度136bp 的特异性片断,21株非新生隐球菌未见此片断。扩增灵敏度为10cfu。临床标本的 PCR 检测结果为:23份涂片法或(和)真菌培养阳性的脑脊液标本扩增也均为阳性(100%),14份涂片法和真菌培养阴性的脑脊液标本13份扩增阴性(93%)。认为rDNA 聚合酶链反应是诊断隐球菌性脑膜炎的一种简便快速、灵敏、特异的方法。
Polymerase chain reaction(PCR) was used for the first time in China for the diagnosis of cryptococcal meningitis by amplification of specific sequence of the rDNA genes of C.neoformans.All 11 strains of C.neoformans yielded a specific 136 bp fragment but 21 non-C,neoformans strains did not. The sensitivity of the amplification was about 10 cfu.With the aid of the rDNA-PCR,23 of the 23 cere- brospinal fluids (CSF) specimens which had been confirmed C.neoforrnans positive by smear and/or cul- ture were PCR-positive(100%),and 13 of the 14 CSF specimens which had been confirmed C.neofor- mans negative by smear and culture were PCR-negative (93%).The results of the present study suggest that rDNA-PCR is a sensitive and specific method for rapid diagnosis of cryptococcal meningitis.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1996年第5期319-321,共3页
Chinese Journal of Dermatology
基金
中华医学会皮肤科学会科研基金赞助项目