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重组人内抑素对佐剂性关节炎大鼠滑膜组织血管内皮细胞生长因子表达的影响 被引量：13

Effect of recombinant human endostatin on expression of vascular endothelial cell growth factor in adjuvant arthritis synovium tissue
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摘要 目的:观察重组人内抑素对佐剂性关节炎大鼠滑膜组织血管内皮细胞生长因子(vascular endo-thelial cell growth factor,VEGF)的表达及其作用。方法:采用弗氏完全佐剂(Freund’s complete adjuvant,CFA)诱导的佐剂性关节炎(adjuvant-induced arthri-tis,AA)大鼠模型,用足容积法测量关节肿胀度;免疫组织化学法检测血管内皮细胞生长因子的表达及其对微血管密度的影响。结果:弗氏完全佐剂致炎后d 10,佐剂性关节炎大鼠出现继发性炎症,皮下注射给予不同剂量的重组人内抑素(1.25、2.5、5 mg.kg-1),连续7 d,对照组于d 10给予氨甲喋呤(Methotrexate,MTX,1.0 mg.kg-1)。重组人内抑素各剂量组能明显抑制AA大鼠的继发性足肿胀,重组人内抑素各剂量组可不同程度减少AA大鼠关节滑膜组织高表达的VEGF,还可使滑膜组织的微血管密度减少。结论:重组人内抑素可显著抑制AA大鼠滑膜组织VEGF的表达及微血管密度,该作用可能与抑制血管翳的生成及减少滑膜细胞表面VEGF的表达有关。 AIM：To investigate the effect of recombinant human endostatin on the expression of vascular endothelial cell growth factor （VEGF） in adjuvant arthritis synovium tissue. METHODS： Adjuvant arthritis rat model was induced by a single intradermal injection of 100 μl of Freund＇ s complete adjuvant（CFA）on d 0. The volume of rat hind paw was measured by means of Volume Meter. The expression of VEGF and microvessel density of the synovial tissue were measured by immunohistochemical method. RESULTS： The secondary inflammation of Adjuvant-induced arthritis（AA） rats appeared on the d 10 after the injection of CFA. The therapeutic administration of recombinant human endostatin（ 1.25,2.5,5.0 mg·kg^-1 ） were given for 7 days consecutively. Methotrexate （MTX） （1.0 mg·kg^-1）was given only once on d 10 as the control. It was found that recombinant human endostatin significantly inhibited the secondary paw swelling. Recombinant human endostatin significantly decreased the expression of VEGF and microvessel density of the synovial tissue on AA rots. CONCLUSION： Recombinant human endostatin had an inhibitory effect on the expression of VEGF and microvessel density of the synovial tissue on AA rats, which was related to its anti-angiogenesis and an inhibition of VEGF on synoviocytes.
作者 夏丽娟 陈飞虎 黄学应 刘永靖 李俊
机构地区 安徽医科大学药学院
出处 《中国临床药理学与治疗学》 CAS CSCD 2006年第7期760-763,共4页 Chinese Journal of Clinical Pharmacology and Therapeutics
基金 国家自然科学基金资助项目(№30572196)
关键词 重组人内抑素 佐剂性关节炎 血管内皮细胞生长因子 微血管密度 recombinant human endostatin adjuvant arthritis vascular endothelial cell growth factor microvessel density
分类号 R965 [医药卫生—药理学] R966 [医药卫生—药理学]
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参考文献14

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  • 2O'Reilly MS,Boehm T,Shing Y,Fukai N,Vasios G,Lane WS,et al.Endostatin:an endogenous inhibitor of angiogenesis and tumor growth[J].Cell,1997;88:277-85
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二级参考文献30

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共引文献22

同被引文献169

引证文献13

二级引证文献94

中国临床药理学与治疗学
2006年 第7期

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