摘要
目的:探讨中药护肝灵对乙醇代谢相关酶的影响.方法:以乙醇灌胃法复制大鼠模型,分别以易善复及高、低剂量护肝灵处理,双波长法检测血中乙醇浓度,按试剂盒说明书检查肝匀浆中CAT含量,活性分光光度法检测肝匀浆ADH含量,荧光实时定量RT-PCR观察ADH和CYP450ⅡE1的mRNA表达.结果:与正常对照组相比,模型组血清乙醇含量显著升高(545.7±l08.85mg/L vs -51.4±2.83mg/L,P<0.05),ADH和CAT含量显著降低(667±272nkat/L vs 1884±650nkat/L,P<0.05:112±29nkat vs 255±43nkat,P<0.05).与模型组相比,中药护肝灵可以降低口服乙醇大鼠血中乙醇含量(低剂量:248.5±96.63mg/L;高剂量:-0.1±33.94mg/L;均P<0.01),提高肝组织ADH(低剂量:1184±422nkat/L:高剂量:1550±458nkat/L;均P<0.05)和CAT(低剂量:202±31nkat/L;高剂量:167±34nkat/L;均P<0.05)含量,造模后肝组织CYP450ⅡE1 mRNA显著下降(10±2 vs 15±2,P<0.01),护肝灵可使肝组织CYP450ⅡE1 mRNA表达进一步下降(高剂量:8±2,P<0.05).造模及药物处理后,ADH mRNA均无显著变化(P>0.05).结论:中药护肝灵可激活乙醇代谢相关酶,进而促进乙醇在体内的代谢.
AIM: To investigate the effects of Hu Gan Ling on lytic enzymes of ethanol in the experimental rats. METHODS: The rat model of liver injury was induced intragastically by alcohol administration. After treatment with Essentiale, high- and low-dose Hu Gan Ling, the alcohol concentration in serum was determined by dual wavelength spectrophotometry, and the contents of catalase (CAT) and alcohol dehydrogenase (ADH) in liver homogenate were detected according to the instruction of kit and by spectrophotometric method, respectively. The expressions of ADH and CYP450 Ⅱ E1 mRNA were examined by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: In comparison with that in the normal controls, the serum content of alcohol in model group was significantly increased (545.7 ± 108.85 mg/L vs -51.4 ± 2.83 mg/L, P 〈 0.05), but the tissue content of ADH or CAT was significantly decreased (667 ± 272 nkat/L vs 1884 ± 650 nkat/L, P 〈 0.05; 112 ± 29 nkat vs 255±43 nkat, P 〈 0.05). However, Hu Gan Ling reduced the serum content of alcohol (low dose: 248.5 ± 96.63 mg/L; high dose: -0.1 ± 33.94 mg/L; both P 〈 0.01), but increased the tissue content of ADH (low dose: 1184 ± 422 nkat/L; high dose: 1550 ± 458 nkat/L; both P 〈 0.05) or CAT (low dose: 202 ± 31 nkat/L; high dose: 167 ± 34 nkat/L; both P 〈 0.05). After modeling, the expression of CYP450 Ⅱ E1 mRNA was significantly down-regulated (10 ± 2 vs 15 ± 2, P 〈 0.01), and then further down-regulated by Hu Gan Ling treatment (high dose: 8 ± 2, P 〈 0.05). The mRNA expression of ADH was not markedly changed after modeling and drugs treatment (P 〉 0.05).CONCLUSION: Hu Gan Ling can activate the lytic enzymes of ethanol, and enhance the intracorporal ethanol metabolism.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第21期2077-2081,共5页
World Chinese Journal of Digestology
关键词
护肝灵
分解酶
乙醇
大鼠
Hu Gan Ling
Lyric enzymes
Ethanol
Rats