摘要
利用碘-碘化钾法、氯化三苯基四氮唑(TTC)法及改进TTC法、无机酸法、荧光染色法、离体萌发法、活体萌发法测定了菊属Dendranthema植物菊花脑Dendranthemanankingense,南京野菊Dendranthema indicum,毛华菊Dendranthema vestitum和栽培小菊品种rm43-2,rm46-1,rm48-2的花粉生活力。结果表明:碘-碘化钾法、TTC法及改进TTC法、无机酸法和荧光染色法都不适合菊属植物花粉生活力的检测;活体萌发法检测菊属植物花粉生活力不准确,但能有效表述花粉萌发力;离体萌发法能有效快速地测定花粉生活力,适合菊属植物花粉生活力的检测。
The pollen viability of Dendranthema indicum, D. vestitum, D. nankingense and three cuhivars of chrysanthemum with small infloreseenees (rm43-2, rm46-1, rm48-2) were detected by methods of iodine-iodide kalium, triphenyhetrazolium chloride (TTC) or modified TTC, FDA, mineral acid, germination in vitro and in vivo. The results showed that the methods of iodine-iodide kalium, TIC or modified TIC, FDA, mineral acid were not able to detect the pollen viability of chrysanthemum. The method of germination in vivo can not test the pollen viability but describe the pollen vigor. The method of germination in vitro was fit for detection of pollen viability of chrysanthemum. [Ch, 1 fig. 11 ref.]
出处
《浙江林学院学报》
CSCD
北大核心
2006年第4期406-409,共4页
Journal of Zhejiang Forestry College
基金
国家自然科学基金资助项目(30400308)
江苏省教育厅高技术产业化研究项目(JH02-086)
江苏省科学技术厅高技术研究项目(BG2003305)
上海市农业委员会重点攻关项目[(2004)D3-1]
关键词
植物学
栽培小菊
菊属植物
花粉活力
检测方法
botany
chrysanthemum cuhivars with small inflorescences
Dendranthema
pollen viability
detection