黄芩苷对CPn感染高胆固醇饮食小鼠血清TNF-α、IL-6、IL-10的调节作用

The Influence of Baicalin on the level of Serum TNF-α,IL-6,IL-10 in Diet-induced Hyperlipidemic and CPn-infected Mice

目的:探讨肺炎衣原体感染高胆固醇饮食小鼠后血清TNF-α、IL-6、IL-10水平的变化及黄芩苷的治疗作用。方法:小鼠随机分为黄芩苷高剂量组、黄芩苷低剂量组、阿奇霉素组、高胆固醇+CPn感染组、正常对照组。喂饲高胆固醇饲料1周后,经一侧鼻腔吸入含CPn的培养液,每周1次,共3次。最后一次接种5天后开始给药至实验结束,第1次接种后第18周处死全部动物。结果:高胆固醇+CPn感染组血清TNF-α、IL-6、IL-10水平不同程度升高,三个治疗组三种炎症因子水平不同程度下降。结论:CPn感染可使高脂饮食C57BL/6J小鼠血清TNF-α、IL-6、IL-10水平升高,早期给予高、低剂量黄芩苷或阿奇霉素治疗可不同程度地调节这三种炎症因子的水平。

Objective： To study the variation of serum TNF-α, IL-6, IL-10 in diet-induced hyperlipidemic and chronic CPn-infected mice and the intervention of baicalin. Methods： Eight-week-old SPF male C57BL/6J mice were divided randomly into 6 groups： high-dosage-balcalin group （group Ⅰ ）, low-dosage-baicalin group （group Ⅱ ）, azithromycin group （group Ⅲ）, cholesterol ＋ CPn group （group Ⅳ ）, and normal group （group Ⅴ ） .A week after being fed cholesterol-supplemented diet, group Ⅰ , group Ⅱ , group Ⅲ , group Ⅳand group Ⅴ were inoculated with CPn at 0.5 - 1 × 10^7 IFU per inoculation. Each mouse of these groups was inoculated once weekly for 3 consecutive weeks. Administration started 5 days after the last inoculation. Results： The level of serum TNF-α, IL-6 and IL-10 in group Ⅳ increased. All the three treatment groups declined in different extent. Conclusions： CPn infection can raise the level of serum TNF-α and IL-6 in diet-induced hyperlipidemic C57BL/6J mice. Early treatment of high-dosage-baicalin, low-dosagebaicalin and azithromycin can lower them in some extent.