初发非霍奇金淋巴瘤不同中医证型间免疫状态的比较

Study on immune state of initial NHL patients with different types of TCM syndrome differentiation

目的比较非霍奇金淋巴瘤(NHL)患者不同中医证型间T细胞亚群和NK细胞比例的差别,旨在探讨NHL中医临床辨证分型的客观化检测指标。方法依据中医辨证分型方法,选取寒痰凝滞型NHL21例和阴虚火旺型NHL17例,同时采用流式细胞仪检测患者外周血淋巴细胞中Th、Ts/Tc及NK细胞阳性率,并比较不同中医证型间上述细胞阳性率的差别。结果寒痰凝滞型患者外周血NK细胞阳性率与阴虚火旺型差异无显著意义(P>0.05),外周血Th细胞阳性率较阴虚火旺型患者显著降低(P<0.05),Ts/Tc细胞阳性率较阴虚火旺型患者明显升高(P<0.05)。结论检测恶性淋巴瘤患者Th、Ts/Tc及NK细胞阳性率,可作为中医辨证分型的客观化指标。NHL患者的免疫功能状态也是影响生存质量及生存期的重要因素之一。

Objective To compare the positive ratio of Th, NK and Ts/Tc cells between two types of TCM syndrome differentiation for NHL patients, and try to uncover the objective parameter for syndrome differentiation of NHL clinical diagnosis.Methods According to TCM,21 patients with stagnant cold phlegm and 17 patients with hyperactivity of fire caused by deficiency of yin were selected. Meanwhile, the positive ratio of Th, NK and Ts/Tc cells in peripheral blood lymphocytes were detected by flow cytometry. Then the relations between syndrome differentation types and positive ratio of Th, NK and Ts/Tc cells were compared. Results Although there was no significant difference between two type for positive ratio of NK cells （ P 〉 0.05）,but Th positive ceils in patients with stagnant cold phlegm were lower than those in patients with hyperactivity of fire caused by deficiency of yin （ P 〈 0.05） ; and the ratio of Ts/Tc positive cells in patients with stagnant cold phlegm was higher than the another type （ P 〈0.05）. Conclusion The ratio of positive Th, NK and Ts/Tc cells may be the objective paramenter for clinical syndrome differentiation of NHL patients. Immune state is one of the most important factors determining living quality and life span of NHL patients.