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低浓度洗涤血小板促进人牙髓细胞增殖的作用机制探讨 被引量:3

Washed platelets at low concentration promote the proliferation of human dental pulp cells
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摘要 目的:探讨低浓度洗涤血小板促进人牙髓细胞增殖的作用机制。方法:使用从成年男性志愿者静脉采集、制备的洗涤血小板(washed platelet,WPLT)作用于人牙髓细胞,人牙髓细胞增殖和产生的前列腺素E2(PGE2)采用细胞和PGE2测定试剂盒测定;表达的细胞内环氧酶-2(COX-2)mRNA采用Realtime RT-PCR方法测定。结果:50ml/LWPLT诱导人牙髓细胞COX-2mRNA的表达在作用后3h达到最高峰,而诱导PGE2的产生快于IL-1β;50ml/LWPLT诱导的人牙髓细胞增殖可以被消炎痛和地塞米松抑制,而添加PGE2可以逆转这种抑制作用;WPLT诱导的PGE2量随着其浓度的成倍增加呈现出明显的增多趋势,而PGE2在适当浓度范围内明显促进了人牙髓细胞的增殖。结论:低浓度WPLT可能通过诱导产生的PGE2促进了人牙髓细胞的增殖。 Objective:To investigate the effects of washed platelets at low concentration (WPLT) on the proliferation of human dental pulp cells (HDPCs). Methods : WPLT was prepared from a male venous blood, cultured HDPCs were exposed to the WPLT at 50 ml/L, 100 ml/L and 200 ml/L for 0.5 - 24 h respectively. Cell proliferation and prostaglandin E2 production were examined by cell counting and Prostaglandin E2 EIA Kit-Monoclonal respectively. Cyclooxygenase-2(COX-2) mRNA expression was determined by real time RT-PCR. Results:WPLT at 50 ml/L promoted HDPC proliferation and increased COX-2 mRNA expression at 3-hour-exposure. That at 50 - 200 ml/L increased the production of PGE2 dose-depandently. Indomethacin or dexamethasone reduced WPLT-induced HDPC proliferation, and this inhibitory effect was reversed by exogenous PGE2. PGE2 promoted HDPC proliferation at 5 - 100 ng/ml. Conclusion: WPLT may induce prostaglandin E2 production, thereby promote HPDC proliferation.
出处 《实用口腔医学杂志》 CAS CSCD 北大核心 2006年第5期675-678,共4页 Journal of Practical Stomatology
关键词 人类 牙髓 细胞 血小板 前列腺素E2 细胞内环氧酶-2 Human Dental pulp Cell Blood platelets Prostaglandin E2 Cyclooxygenase-2
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参考文献11

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二级参考文献17

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共引文献11

同被引文献65

  • 1段建民,菊地宽高,汪维健,片山直.血小板血浆对人牙髓细胞增殖的影响[J].牙体牙髓牙周病学杂志,2006,16(4):188-191. 被引量:12
  • 2李威,卢友光,苏柏华,丁林灿,闫福华.富血小板血浆复合MTA盖髓的组织学观察[J].口腔医学研究,2006,22(5):478-481. 被引量:8
  • 3Kawase T, Okuda K, Wolff LF, et al. Platelet-rich plasmaderived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteoblastic cells in vitro[ J]. J Pe- riodontol, 2003, 74(6): 858-864.
  • 4Sanchez AR, Sheridan PJ, Leo L, et al. Is platelet-rich plasma the perfect enhancement factor? A current review [ J ]. Int J Oral Maxillofac Implants, 2003,18 ( 1 ) : 93 - 103.
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  • 6Choi BH, Zhu SJ, Kim BY, et al. Effect of platelet-rich plasma(PRP) concentration on the viability and proliferation of alveolar bone cells: An in vitro study[ J ]. Int J Oral Maxillofac Surg, 2005, 34(4): 420-424.
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