细胞色素c在单壁碳纳米管表面的固定、直接电子转移及电催化

Immobilization of Cytochrome c on the Surface of Single-wall Carbon Nanotube and Its Direct Electron Transfer and Electrocatalysis

应用吸附法将细胞色素c(Cytoc)固定在单壁碳纳米管(SWNT)表面.红外光谱(IR)显示被固定的Cytoc能保持原有的空间结构,没有发生变性.循环伏安测试表明,Cytoc在SWNT表面能发生稳定的直接电子转移,其i^E曲线上出现一对良好的、几乎对称的氧化还原峰.式量电位E0’基本不随扫速的增加而变化(在20 mV^120 mV/s的扫速范围内,E0’平均值为0.165±0.001V).实验同时给出,吸附在SWNT表面的Cytoc仍能保持其对H2O2电化学还原的生物电催化活性.

Cytochrome c （Cyto c） was immobilized on the surface of the single-wall carbon nanotube （SWNT） by using the method of adsorption. Infrared spectroscopy indicated that the Cyto c remained in its original structure and did not undergo structural change after its immobilization on the SWNT. The direct electrochemistry of cyto c, which was adsorbed on the surface of the SWNT, was studied by cyclic voltammetry. The voltammctric results demonstrated that the SWNT had promoting effects on the direct electron transfer of Cyto c and also indicated that the immobilized Cyto c retained its bioelectrocatalytic activity to the reduction of H2O2. This modified electrode might be used in development of new biusensors and the biofuel cells.