摘要
目的:探讨凋亡抑制因子Survivin在食管鳞癌(ESC)组织中的表达及其肿瘤细胞进行体视学分析．方法:应用免疫组织化学SP法检测52例不同分化食管鳞癌Survivin蛋白的表达,运用图像分析系统在显微镜下测量Survivin阳性肿瘤细胞的体视学参数,依据有关体视学公式计算出参数核的体密度(Vvn)、核的表面积密度(Svn)、核的平均体积(V)、核的平均表面积(S)、核的平均自由程(λ)、核的数密度(Nv)、核的平均曲度密度(Kv),比较这些参数在不同分化肿瘤的差异．结果:Survivin蛋白在ESC组织中阳性表达率为57．7％,其表达越强,组织分化程度越低(X2 =6．1099,P=0．0471)．参数Vvn,Svn,V,S的大小是依高、中、低分化的顺序增加,而Kv, Nv,λ九依高、中、低分化的顺序减小,其中Vvn (F=3．8368,P=0．0342),Svn(F=3．7143,P= 0．0376)参数有显著性差异(P<0．05)结论:ESC组织Survivin蛋白的表达与其分化程度有密切关系,肿瘤细胞Vvn,Svn参数是肿瘤细胞分化重要指标,且为临床形态学诊断提供了依据．
AIM： To investigate the expression of Survivin in esophageal squamous carcinomas （ESC） and measure the stereology parameters of ESC cells.
METHODS： SP immunohistochemistry was used to detect the expression of Survivin protein in 52 ESC patients. Thirty Survivin-positive ESC cells were measured with image analyze system under microscope. The data for the stereological analysis include the body density of nucleus （Vvn）, the surface area density of nucleus （Svn）, the mean volume of nucleus （V）, the surface area of nucleus （S）, the mean free path of nucleus （λ）, the number density of nucleus （Nv）, and the mean curvature density of nucleus （Kv）. The differences of the parameters were compared among the tumors with different degrees.
RESULTS： The positive rate of Survivin protein was 57.7% in ESC, and up-regulated expression of Survivin was strongly associated with poor differentiation （X^2 = 6.1099, P = 0.0471）. The values of Vvn, Svn, V and S were increased, but those of Kv, Nv, and λ were decreased ordinally in well, moderately and poorly differentiated tu- mor cells, of which the values of Vvn （F = 3.8368, P = 0.0342） and Svn （F = 3.7143, P = 0.0376） had significant differences （P 〈 0.05）.
CONCLUSION： The expression of Survivin protein is correlated with the differentiation in ESC. Vvn and Svn were important indexes of cellular abnormity, which provides evidences for clinical morphological diagnosis.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第23期2315-2318,共4页
World Chinese Journal of Digestology
