HPLC指纹图谱结合系统聚类法对不同产地关黄柏药材的分析研究

Studies of Cortex Phellodendri Amuren on HPLC Fingerprint and Genealogied Cluster Analysis

目的:建立关黄柏药材HPLC指纹图谱,为其质量控制提供有效的方法。方法:HPLC法,Hypersil-C18反相色谱柱,A相为甲醇,B相为0.3%磷酸二乙胺水溶液,梯度洗脱,检测波长为230nm;系统聚类法,对关黄柏样品的HPLC-FP中各特征峰的相对峰面积进行分析。结果:20批关黄柏样品指纹图谱相似度均在0.80以上,其中道地药材样品质量较好。结论:该方法稳定可靠,可以有效地用于关黄柏药材的质量评价。

An effective and convenient method was established for HPLC fingerprints of Cortex Phellodendri Amuren, which provided an approach for quality control. Methods： Hypersil-C18 column, phase A： meth, phase B：0.3%, eluted with a gradient program and detected at 230 nm. Genealogied cluster analysis characteristic absorption band.Results： The fingerprint method for Cortex Phellodendri Amuren was established. The similarity of 20 batches of Cortex Phellodendri Amuren was not lower than 0.80. Conclusion： This validated method is available for quality evaluation and quality control in Cortex Phellodendri Amuren.