海湾扇贝外套膜的组织结构与粘液细胞的类型与分布

Microscopic Anatomy and Types of Mucous Cells of the Mantle of Argopectens irradians

运用HE及AB-PAS染色方法,在光镜下对海湾扇贝(Argopectens irradians)外套膜的组织学结构及粘液细胞的类型与分布进行了观察。结果表明,该部位具有双壳贝类外套膜的典型结构特征,即由中央膜和边缘膜组成,边缘膜具有3个突起,分别为生壳突起、感觉突起和缘膜突起。组织结构主要包括内外上皮层、结缔组织与肌纤维。不同部位外套膜各组织成分的含量与分布有差异。AB-PAS染色反应显示,在上皮层中分布有几种不同类型的粘液细胞,分别为Ⅰ、Ⅱ、Ⅲ、Ⅳ型。Ⅰ型着色为红色,AB阴性,PAS阳性,含中性粘多糖;Ⅱ型为蓝色,AB阳性,PAS阴性,含酸性粘多糖;Ⅲ型染色后呈紫红色,AB,PAS均为阳性,PAS阳性较强,含混和性粘多糖,以中性粘多糖为主;Ⅳ型为蓝紫色,AB,PAS均呈阳性,AB阳性较强,含混和性粘多糖,以酸性粘多糖为主。不同部位粘液细胞的类型与数量均有差异。

The microscopic anatomy and the different types of mucous cells in the mantle of the scallop, Argopectens irradians found from Qingdao, China are described using methods of hematoxylin and Eosin Y staining and AB-PAS （alcian blue and periodic acid Schiff＇s reaction）combined procedure. The typical structure of the mantle of bivalve consisting of a middle zone and a marginal zone is observed. Three protuberances are observed in the marginal zone. There are shell protuberance, sensory protuberance and velum protuberance. The mantle is composed of inner epithelium, outer epithelium, connective tissue and muscular fiber. Different types of mucous cells are also detected in epithelium. Based on our observation, the mucous cells can be divided into four types after staining： type Ⅰ, pure red, PAS positive and AB negative, cells containing neutral mucopolysaccharide; type Ⅱ, blue, PAS negative and AB positive, with acid mucopolysaccharide; type Ⅲ, AB positive more than PAS, mixed mucopolysaccharide can be observed; type Ⅳ, PAS positive more than AB, possessing also mixed mueopolysaocharide. All four types of mucous cells are located in different parts of the mantle.