抗原致敏DC与CIK共培养体外抗肾癌效应的研究

Effects of dendritic cells co-cultured with CIK cells on renal carcinoma cells

目的:探讨肾癌(RCC)抗原致敏树突状细胞(DC)与同源细胞因子诱导杀伤细胞(C IK)共培养后的DC-C IK细胞对RCC的杀伤活性。方法:将健康成人外周血单个核细胞(PBMC)来源的DC经RCC(786-0细胞株)抗原致敏后与同源C IK细胞共培养,实验分3组:RCC抗原致敏DC与C IK共培养组(A组),未致敏DC与C IK共培养组(B组),单纯C IK组(C组)。流式细胞仪检测DC及C IK免疫表型。MTT法检测3组效应细胞对786-0细胞杀伤活性。结果:效靶比20∶1时,A、B、C组对786-0细胞杀伤活性分别为(70.64±8.26)%、(53.40±7.33)%、(46.64±6.01)%,各组比较差异显著(P<0.05);以前列腺癌PC3细胞作靶细胞对照,A组对786-0及PC3细胞的杀伤活性有显著差异(P<0.05)。结论:RCC抗原致敏DC与C IK共培养后的DC-C IK细胞可明显提高C IK细胞对RCC的杀伤特异性和杀伤活性。

AIM ： To study the effects of CIK cocultured with DC that pulsed with RCC antigen on renal carcinoma cells. METHODS ： DC and CIK cells were generated respectively by cytokines from PBMC of healthy blood donor. Cell surface markers were analyzed by flow cytometry. Then CIK were cocultured with autologous DC that was （or not） pulsed with RCC antigen （786 -0 cells）. Cytotoxic activity against 786 -0 or PC3 cells was measured by MTT assay under three different conditions ： CIK cocultured with DC which was pulsed with 786 - 0 antigen （ group A） ; CIK cocultured with DC which is not pulsed with 786 - 0 antigen （group B） ; CIK without DC （ group C）. RESULTS ： The cytotoxic activity of three groups against 786 -0 cells was （70. 64 ±8. 26）%, （53.40 ±7. 33）%, （46. 64 ±6. 01 ）%, respectively （E/T =20：1 ）. Significant differences between group A and group B or between group A and group C were observed （ P 〈 0. 05）. There was a significant difference in cytotoxic effects of group A against 786 -0 and PC3 cells （P 〈0.05）. CONCLUSION： Coculture of CIK with autologous tumor - pulsed DC leads to a significant increase in cytotoxic activity against renal carcinoma cells, and cytotoxicity mediated by RCC lysate antigen possess stronger specificity.