摘要
目的:观察酒精性肝纤维化大鼠Hsc和肝细胞的凋亡及中药清肝活血方对细胞凋亡的影响.方法:以乙醇为主制备酒精性肝纤维化大鼠模型,将造模大鼠分为清肝活血方低(4.75g/kg)、中(14.25g/kg)和高剂量组(28.5g/kg),每日进行ig药物干预2wk,并设空白对照组、模型对照组及易善复对照组.比色法检测血清ALT,AST,γ-GT;HE和Masson染色观察肝组织炎症和纤维化程度.TUNEL检测肝细胞凋亡,TUNEL-α-SMA双标记检测HSC凋亡.结果:清肝活血方用药组及易善复组能降低大鼠血清ALT(1213±245,1432±253nkat/Lvs2140±428nkat/L,P均<0.05),AST(1671±400,2123±413vs4454±850nkat/L,P均<0.05),γ-GT水平(4539±1847,5509±2430vs8271±3304nkat/L,P均<0.05),减轻纤维化程度(5.5±2.50,6.30±3.16vs9.00±2.27,P<0.05);诱导活化的HSC凋亡(5.25%±2.48%,3.63%±2.04%vs2.30%±1.24%,P<0.05),减少肝细胞凋亡(0.43%±0.11%,0.60%±0.16%vs1.77%±0.49%,P<0.05).结论:清肝活血方能有效减轻大鼠肝纤维化程度,并减少乙醇引起的肝细胞凋亡,诱导活化的HSC凋亡.
AIM: To evaluate the influences of Qinggan Huoxue recipe (QHR) on the apoptosis of hepatic stellate cells (HSC) and hepatocytes in alcoholic liver fibrosis rats.
METHODS: Rat model of alcoholic liver fibrosis were established mainly through alcohocol, and then the rats were divided into 6 groups: QHR groups [low dose: 4.75 g/(kg.d), moderate dose: 14.25 g/(kg'd), high dose: 28.5 g/(kg.d)] and control groups (normal, model, and Essentiale control). Colorimetry was used to detect the serum levels of alanine aminotransferase (ALT), aspartate transaminase (AST) and gammaglutamyltranspeptidase (γ-GT). The degrees of inflammation and fibrosis were evaluated by HE and Masson stainings. TUNEL and TUNEL-α- SMA methods were used to detect the apoptosis of HSC and hepatocytes, respectively. RESULTS: As compared with those in model control group, the serum levels of ALT (1213± 245, 1432 ± 253 nkat/L vs 2140 ± 428 nkat/L, both P 〈 0.05), AST (1671 ± 400, 2123 ± 413 nkat/L vs 4454 ± 850 nkat/L, both P 〈 0.05), and γ-GT (4539 ± 1847, 5509 ± 2430 nkat/L vs 8271 ± 3304 nkat/L, both P 〈 0.05) were significantly decreased in QHR (high dose) group and Essentiale control group; the apoptosis rates of hepatocytes were reduced (0.43% ± 0.11%, 0.60% ± 0.16% vs 1.77% ± 0.49%, both P 〈 0.05) and the apoptosis rates of HSC were increased (5.25% ± 2.48%, 3.63% ± 2.04% vs 2.30% ± 1.24%, both P 〈 0.05); the degrees of inflammation and fibrosis were relieved too (5.5 ± 2.50, 6.30 ± 3.16 vs 9.00 ± 2.27, P 〈 0.05).
CONCLUSION: Chinese medicine QHR can relieve alcohol-induced fibrosis, decrease apoptosis of hepatocytes and increase apoptosis of HSC.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第26期2590-2595,共6页
World Chinese Journal of Digestology
基金
上海市科委启明星跟踪计划项目
No.03QMH1410~~
关键词
清肝活血方
酒精性肝纤维化
肝星状细胞
凋亡
Qinggan Huoxue recipe
Alcoholic liverfibrosis
Hepatic stellate cell
Apoptosis