白亮独活的组织培养和植株再生研究

Research on the Tissue Culture and Plantlet Regeneration of Heracleum candicans

建立了白亮独活组织培养和小植株再生的体系。以叶片为外植体,在含不同生长调节物质的MS培养基中培养,结果表明,在MS培养基上附加2,4-D 2.0 mg/L和6-BA 0.5 mg/L对愈伤组织的诱导率可达100%;而愈伤组织继代最合适的培养基培养为附加2,4-D2.0 mg/L和6-BA0.2 mg/L的MS培养基;苗的诱导以附加6-BA2.0 mg/L的MS培养基效果最好;生根在附加NAA0.2mg/L和IAA0.5 mg/L的1/2 MS培养基中最好。

A system of tissue culture and plantlet regeneration of Heracleum candicans was established. The different combinations of plant growth regulators were added into MS medium to investigate their effects on tissue culture of leaf as explants and 100% callus was inducted on MS media with 2.0 mg/L 2,4-D and 0.5 mg/L 6-BA. And the most suitable subculture medium was MS with 2,4-D 2.0 mg/L and 6-BA 0.2 mg/L. Shoots were proliferated on MS media supplemented with 6-BA 2.0 mg/L and the rooting medium was 1/2 MS media added with NAA 0.2 mg/L and IAA 0.5 mg/L.