苦瓜多糖对链脲佐菌素诱导糖尿病小鼠的降血糖效果

THE ANTIHYPERGLYCEMIC EFFECTS OF POLYSACCHARIDE FROM MOMORDICA CHARANTIA IN STZ-INDUCED DIABETIC MICE

目的:研究苦瓜水提多糖(watersolublepolysaccharidesofMomordicacharantiaL.MCW)和苦瓜碱提多糖(alkalinesolublepolysaccharidesofMomordicacharantiaL.MCB)对STZ诱导糖尿病小鼠的降血糖作用。方法:昆明种小鼠120只,随机抽取12只作空白对照,余下108只,禁食后于次日晨腹腔注射链脲佐菌素(STZ)200mg/kgbw。取血糖值在11.1mmol/L以上者随机分组,共4组:模型组、MCW组(300mg/kgbw)、MCB组(300mg/kgbw)和优降糖组(15mg/kgbw)。连续灌胃10d后,禁食,次日晨眼眶取血,测定血糖及血清胰岛素含量。解剖取胰腺,观察小鼠胰岛组织形态。结果:MCW和MCB都可以降低糖尿病小鼠空腹血糖,MCB组分的降血糖效果最佳,不但可以显著降低糖尿病小鼠空腹血糖值,而且可以提高糖尿病小鼠血清胰岛素水平。结论:苦瓜多糖能帮助恢复受损胰岛组织,促进胰岛素的分泌,从而降低血糖。

Objective： To study the antihyperglycemic effect of water soluble polysaccharides （MCW） and alkaline soluble polysaccharides （MCB） from Momordica charantia L. on streptozotocin-induced diabetic mice. Method： Twelve mice were selected randomly as control, while the other 108 mice were injected with STZ 200 mg / kg · bw to induce diabetes. The diabetic mice were divided into 4 groups, the model, MCW （300 mg/kg bw）, MCB （300 mg/kg bw）, Gilbenclamide （15 mg/kg bw）. The above components were administered intragastrically for 10 d and then blood samples were taken to determine concentrations of serum glucose and insulin. Results： The blood glucose levels could be obviously decreased by MCW and MCB in the diabetic mice. MCB has the best antihyperglycemic effect, in terms of improving the serum insulin level and decreasing the blood glucose level of diabetic mice. Conclusion： The polysaccharides from Momordica charantia can repair the injured pancreatic islet tissues and enhance insulin secretion so as to reduce blood glucose level of the diabetic mice.