摘要
以成年母猪为研究材料,采用RT-PCR半定量法对猪PPARα、β/δ和γ基因组织表达特点进行了研究。结果表明:在检测的18种组织中除胰腺组织外,3种PPAR亚型在其他17种组织中均有表达。表达量高低依次为PPARα,子宫绒毛膜>皮下脂肪>卵巢>大肠>脾脏>大脑>肾上腺>心脏>肺>小肠>脊髓>子宫蜕膜>胃>肝脏>背最长肌>膀胱>肾脏;PPARδ,子宫绒毛膜>卵巢>大肠>脾脏>肝脏>胃>皮下脂肪>大脑>肺>肾上腺>子宫蜕膜>脊髓>背最长肌>心脏>肾脏>小肠>膀胱;PPARγ,背最长肌>皮下脂肪>卵巢>脾脏>肺>大肠>膀胱>子宫绒毛膜>子宫蜕膜>心脏>胃>肝脏>肾脏>大脑>脊髓>肾上腺>小肠。3种亚型PPAR在卵巢和/或子宫绒毛膜中都有较高的表达,提示它们与猪的繁殖性能相关。
The objective of this study was to determine the expression characteristics of Peroxisome proliferator-activated receptors （PPAR）α, δ/β and y in different tissues of porcine. The results of the quantitative RT-PCR showed that except for pancreas, the mRNA of PPARα, δ/β and y are expressed in all detected tissues. The relative expression level of PPARα was the highert in uterine chorion, subcutaneous fat and ovary, medium in large intestine, spleen, brain, adrenal gland, heart and lung, and the lowest in small intestine, spinal cord, uterine decidua, stomach, liver, longissimus muscle, urinary bladder and kidney. The relative expression level of PPARδ was the highest in uterine chorion, ovary, large intestine, spleen, liver and stomach, medium in subcutaneous fat, brain, lung and adrenal gland, and the lowest in uterine decidua, spinal cord, longissimus muscle, heart, kidney, small intestine and urinary bladder. However, the highest expression level of PPARy was in longissimus muscle, subcutaneous fats, ovary, spleen, lung and large intestine, medium in urinary bladder, uterine chorion, uterine decidua and heart, and the lowest in stomach, liver, kidney, brain, spinal cord, adrenal gland and small intestine. The present study simultaneously suggested that the PPAR genes are associated with the reproductive performance of sows.
出处
《中国畜牧杂志》
CAS
北大核心
2006年第19期1-4,共4页
Chinese Journal of Animal Science
基金
国家高技术研究发展计划项目(2002AA242031)
国家自然科学基金项目(30571322)
