莪术多糖对雏鸡抗氧化能力和免疫功能的影响

Effects of Curcuma zedoaria Rosc.Polyszccharide on Antioxidant Status and Immunity Function in Chickens

采用160只7日龄健康雏鸡,随机分为4组,即正常对照组(Ⅰ)、环磷酰胺组(Ⅱ)、环磷酰胺+莪术多糖组(Ⅲ)、莪术多糖组(Ⅳ),分别测定血细胞的吞噬率、吞噬指数,血清的SOD活性及LPO含量,同时处死雏鸡,测定法氏囊和脾脏指数,观察其组织结构。结果表明,莪术多糖对.OH和O2-有明显的清除作用,最高清除率分别是88.91%和98.5%;SOD活性显著增加,与对照组相比最高可提高145%;LPO含量下降,与对照组相比最大可降低40.95%;各组雏鸡体重从使用药第2周开始快速增长,最大平均周增重为48.75 g/周;莪术多糖可使血细胞的吞噬率及吞噬指数、法氏囊和脾脏的相对重量均明显升高,并能促进法氏囊和脾脏的快速发育;环磷酰胺加莪术多糖组上述指标接近正常对照组,说明莪术多糖能补偿环磷酰胺对雏鸡免疫系统的抑制作用。由此可见,莪术多糖具有一定的抗氧化能力,提高机体免疫力,而且能促进雏鸡生产性能,修复组织损伤。

Effects of Curcuma zedoaria Rosc. polyszccharide on the antioxidant status and the immunity function in chickens were studied in the experiment. One hundred and sixty 7-day-old chickens were randomly divided into four groups, control group （ Ⅰ ）, Cyclophoshamide group （Ⅱ）, Cyclophoshamide plus curcuma zedoaria Rosc group （Ⅲ）, Curcuma zedoaria Rosc. group （Ⅳ）. The phagocytosis ability of blood corpuscle, SOD activity and LPO content of blood serum, and the chickens＇ indexes of bursa F and spleen were determined in the blood of each chicken in every group. At the same time, we also observed the structures of bursa F and spleen. The resuits showed that Curcuma zedoaria Rosc. polyszccharide had an evident effect in eliminating · OH and O2^- , and the biggest eliminating ratio was 88.91% and 98.5% respectively; SOD activity and LPO content were significantly changed, SOD activity was enhanced 145% and LPO content reduced 40. 95%, maximally, comparing with the control group; The weights of all chickens were increased quickly from the second week after treatment, the biggest increase weight was 48.75 g per week; The phagocytosis ratio and index of blood corpuscle, weight and growth speed of bursa F and spleen in group of Curcuma zedoaria Rosc. polyszccharide were increased; The values of antioxidant and immunity functions in the group fed with the cyclophoshamide added Curcuma zedoaria Rosc. polyszccharide were similar to the control group, which it showed that the injury to immune organs by cyclophoshamide could be made up by Curcuma zedoaria Rosc. polyszccharide. All in all, Curcuma zedoaria Rosc. polyszccharide had a antioxidant ability in some degree, could strengthen body immunity , increase growth performance of chickens, repair injury of tissue.