黄芩总黄酮提取及纯化工艺的研究

Extraction and purifiction process of total flavonoid compounds in Scutellaria baicalensis

目的:确定黄芩总黄酮提取及纯化的最佳工艺条件和参数。方法:以黄芩总黄酮和黄芩苷提取转移率作为评价指标,采用正交试验方法进行优选。结果:黄芩总黄酮提取及纯化的最佳工艺为:取黄芩颗粒,加10倍量水,煎煮3次,每次2 h,药液浓度调整至0.25 g(生药)/mL,调pH至2,80℃保温60 m in,50%乙醇沉淀,醇液调pH至2,80℃保温60m in,静置12 h,沉淀用50%乙醇洗至pH为4.0。结论:该工艺合理、可行,适合工业化生产。

AIM： To determine the suitable condition and the optimal parameters for the extraction and purifietion process of total flavonoid compounds in Scutellaria baicalensis. METHODS： It was investigated by orthogonal experiment design of form L9 （3^4） with the extraction percentage of the total flavonoid compounds and baicalin from Scutellaria baicalensis as the standard. RESULTS ： The optimal condition of extraction was with water as much as ten times of the medicine amount and was boiled for 2 h and three times, and the optimal condition of purifiction was as follows ： solution concentration 0.25 g （ crude medicinal materials） /mL, adjusting pH to 2, 80℃ heat preservation up to 60 min, 50% alcohol as precipitant and the precipitated adjusted pH to 4.0. CONCLUSIONS ： The optimal process is rational, practical and adapted to industry production.