摘要
目的:探讨甘麦大枣汤加味方对抑郁症模型大鼠海马信号转导cAMP-蛋白激酶A(PKA)途径的影响。方法:以孤养加慢性不可预见性中等刺激所致抑郁症大鼠为模型,灌服甘麦大枣汤加味方,通过旷场行为测定、糖水消耗试验观察行为变化,放免法测定海马cAMP含量以及原位杂交法测定海马5-HT1A受体、PKAmRNA表达。结果:模型组大鼠垂直活动、水平运动及糖水消耗显著下降,海马cAMP、PKAmRNA表达显著上升(P<0.01)。用药组与模型组比较,水平运动及垂直活动上升,糖水消耗增加,cAMP含量显著下降,PKAmRNA表达显著下降(P<0.01)且呈量效关系。结论:甘麦大枣汤加味方下调抑郁症大鼠海马信号转导cAMP-PKA途径可能是该方纠正抑郁症模型大鼠行为学变化的环节之一。
Objective： To observe effects of supplemented Ganmai Dazao Decoction on cAMP-PKA pathway of hippocampal signal transduetion in rat model of depression. Methods：Depression rat model was established by separation and chronic unpredictable mild stress. 60 rats were randomly divided into 4 groups： normal group,model group,low and high dose groups of Supplemented Ganmai Dazao Decoctions. Behaviors of rats were detected by open-field test and sucrose consumption test,contents of cAMP were detected by radio-immunity assay,and expressions of 5-HT1A and PKA mRNA were detected by original hybridization. Results： Scores of ambulation and rearing,sucrose consumption lowered remarkably in model group, contents of cAMP and expressions of PKA mRNA increased remarkably in hippocampus of model rats （P〈0.01）. Compared with model group, scores of ambulation and rearing, sucrose consumption increased remarkably, contents of cAMP and expressions of PKA mRNA lowered remarkably in groups feed drugs （P〈 0.01）, and the lowering levels were proportional to dosage of the Decoction. Conclusion： Down regulation of cAMP-PKA pathway in hippocampus of rat model of depression by supplemented Ganmai Dazao Decoction maybe a way to correct behavioral changes of rat model of depression.
出处
《上海中医药大学学报》
CAS
2006年第4期73-75,F0003,共4页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
上海市教育委员会E-研究院建设计划项目(E03008)
