脾切除对门脉高压症大鼠CD4、CD8和脾功能的影响

Effects of splenectomy on CD4^+,CD8^+ and spleen function in cirrhotic rats with portal hypertension

目的:研究肝硬化门脉高压症(PHT)大鼠脾次全切除术后CD4、CD8与脾功能的变化。方法:皮下注射60%四氯化碳复制大鼠肝硬化模型。共分5组:正常大鼠组、肝硬化(PHT)组、正常大鼠脾切除组、PHT全脾切除组、PHT脾次全切除组,每组10只。手术后第4周检测各组血常规、肝功能、tuftsin、CD4、CD8等指标。结果:PHT高压症大鼠脾切除术后tuftsin水平[(171±21)ng/Lvs(433±44)ng/L,P<0.01]、CD4/CD8(2.01±0.22vs1.12±0.12,P<0.01)均显著低于PHT组;PHT大鼠脾次全切除组tuftsin[(434±42)ng/Lvs(171±21)ng/L,P<0.01]、CD4/CD8(1.97±0.18vs1.12±0.12,P<0.01)显著高于PHT大鼠脾切除组。肝功能两者无显著差异(P>0.05)。结论:PHT脾次全切组大鼠术后免疫和脾功能比PHT脾全切组大鼠明显改善,肝功能两者无显著变化。

AIM： To investigate the effect of subtotal spleneetomy on the expression of CD4 ^＋ ,CD8 ^＋ and tuftsin in eirrhosie rats with portal hypertension （PHT） . METHODS： Rats liver cirrhosis was induced by subcutaneous injection of 40% CCl4. Fifty rats were randomly divided into five groups （n = 10）. Group A： control rats; group B： PHT rats; group C： normal rats with total spleneetomy; group D： PHT with total spleneetomy and group E： PHT with subtotal spleneetimy. The hepatic function, the expression of CD4 ^＋ , CD8 ^＋ , the ratio of CD4^＋ to CD8 ^＋ and tuftsin were analyzed at the fourth week after treatment. RESULTS： The expression of tuftsin , the ratio of CD4 ^＋ to CD8 ^＋ was significantly decreased in PHT rats with total splenectomy compared with PHT rats [ （ 171 ± 21 ） ng/L vs （433 ± 44 ）ng/L,P 〈 0. 01 ; （2. 01 ± 0. 22 vs 1.12 ±0. 12） ,P 〈0. 01 ]. In the group of PHT rats with subtotal spleneetomy, the expression of tuftsin, the ratio of CD4^＋ to CD8^＋ was higher than those in the PHT rats with total splenectomy [ （434 ±42） ng/L vs （ 171 ± 21 ） ng/L,P 〈0.01 ; （ 1.97 ±0. 18 vs 1.12 ±0. 12,P 〈0. 01 ], however, the hepatic function was not show difference（ P 〉 0. 05 ）. CONCLUSION ： Spleen and immune function is significantly improved in PHT rats after subtotal splenectomy, but the hepatic function is not changed significantly.