摘要
目的测定川芎二氧化碳超临界流体萃取物中丁烯基苯酞的含量。方法采用HPLC法。色谱条件为色谱柱:Diamonsil C18(200mm×4.6mm,5μm);流动相:甲醇-体积分数为10%异丙醇水溶液(体积比为60:40);流速:1.0mL·min^-1;检测波长:235nm;柱温:40℃。结果丁烯基苯酞在7.28~72.8μg·mL^-1内线性关系良好(r=0.9995),平均回收率为99.7%(RSD=2.72%,n=6)。3批样品中丁烯基苯酞的含量质量分数分别为4.86%(RSD=1.25%,n=3)、5.00%(RSD=1.87%,n=3)和4.81%(RSD=1.87%,n=3)。结论HPLC法可以测定川芎二氧化碳超临界流体萃取物中丁烯基苯酞的含量。供试品中丁烯基苯酞与其他组分的色谱峰分离度良好。
Objective To determine the content of butylidenephthalide in Rhizoma chuanxiong CO2 supercritical fluid extracts by reversed-phase HPLC method. Methods The sample was separated on a C18 column with the mobile phase of methanol-10 % isopropanol in water solution( V : V = 60 : 40). The flow rate was 1.0 mL'min-! and the detection wavelength was set at 235 nm. The column temperature was 40℃. Results The calibration curve was linear in the range of 7.28 -72.8μg· mL^-1 for butylidenphthalide( r = 0. 999 5). The average recovery was 99.7 % (RSD = 2.72 %, n = 6). The contents of butyllidenephthalide in three batches of the Rhizoma chuanxiong CO2 supercritical fluid extracts were 4.86 % (RSD = 1.25 %, n = 3),5.00% (RSD = 1.87%, n = 3)and 4.81% (RSD = 1.87%, n = 3), respectively. Conclusions The method is simple and accurate, it can be used as a method of quality control for products containing butylidenephthalide.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2006年第12期788-790,共3页
Journal of Shenyang Pharmaceutical University