血清HBV标志物阴性肾炎肾组织中HBV-DNA的原位杂交检测

Detection of HBV-DNA in kidneys of nephritic patients with negative serum HBV markers using in situ hybridization

目的对血清HBV标志物阴性肾炎患者肾组织进行HBV-DNA的检测,为临床诊疗提供理论依据。方法应用原位杂交(ISH)技术检测37份血清HBVAg阴性和18份血清HBVAg阳性的肾炎患者肾活检石蜡包埋组织切片中的HBV-DNA。结果在血清HBVAg阳性的18例标本中HBV-DNA的检出率为88.9%(16/18),血清HBVAg阴性37例中检测出HBV-DNA5例(13.5%)。原位杂交显示两组检出的HBV-DNA均以肾小管上皮细胞质分布为主,与组织中HBVAg阳性颗粒的分布基本一致。结论血清HBVAg阴性的HBV相关性肾炎临床上并非少见,应给予足够的关注。HBV-DNA在肾组织中的检出,表明HBV在HBV相关性肾炎的发病机理中有着重要作用。

Objective To detect the HBV-DNA in kidneys of serum HBVAg markers negative nephritic patients, and to provide theoretical evidence for clinical diagnosis and therapy. Methods To detect HBV-DNA in paraffin-embedded renal biopsy tissue from 18 nephritic patients with positive serum HBV markers and 37 cases with negative serum HBV markers by using in situ hybridization（ ISH）. Results The positive rate of HBV-DNA in renal biopsy was 88.9% （16/18） in patients with positive serum HBV markers. HBV-DNA was found in 5 cases （5/37, 13.5%） of negative serum HBV markers. HBV-DNA was detected mainly in the cytoplasm of tubular epithelia with ISH. The distribution of HBV-DNA was consistent with that of HBVAg. Conclusion HBV associated nephritis patients with negative serum HBV markers are not very uncommon in clinic, which should be paid a great attention. The detection of HBV-DNA in renal tubular epithelia showing an important role of HBV in the pathogenesis of HBV associated glomerulonephritis.