伊莎笼养蛋鸡胰岛素样生长因子I限制性片断多态性与骨矿的研究

Primary study on relationship between bone mass and RFLPs of the insulin-like growth factor-Ⅰgene in ISA layers

选择58周龄高产期伊莎笼养蛋鸡90羽,应用PCR扩增鸡IGF-I启动子上游7 kb处基因片段,用PstI消化产生限制性片段长度多态性,分析IGF-I多态性与胫骨、股骨、肱骨骨密度之间的关系。结果表明,伊莎蛋鸡PP、Pp、pp基因型频率分别为0.256、0.311、0.433,P和p等位基因频率分别为0.411 1和0.588 9。方差分析显示,肱骨的骨密度与IGF-I基因多态性相关,PP基因型组的骨密度和骨矿含量均显著大于pp基因型(P<0.05),股骨、胫骨基因型间无显著差异。PP基因型体质量显著大于Pp基因型。表明伊莎笼养蛋鸡IGF-I基因多态性和肱骨骨密度显著相关,P等位基因具有一定的优良骨质指针作用。

The relationship between restrict on fragment length polymorphism （RFLP） and bone mass was studied in ISA layers. Ninety peak productivity hens were chosen, and the blood samples were collected for DNA extraction, IGF-Ⅰ gene fragment was amplified by PCR, followed by PstⅠ digestion. Three genotypes （PP, Pp, pp） were detected, with the frequencies of PP, Pp and pp genotype being 0.256, 0.311, 0.433, respectively, and the gene frequencies for P and p alleles being 0.411 1 and 0.588 9, respectively. Statistical analysis （ANOVA） showed that there was certain correlation between the bone mineral density （BMD） of humerus and IGF-Ⅰ genotype, BMD and bone mineral content （BMC） of humerus of PP genotype were obviously higher than those of pp genotype （P 〈 0.05）. The body weight of PP genotype was heavier than that of Pp genotype. It could be concluded that there was obvious relationship between polymorphism in IGF-Ⅰ gene and BMD of humerus. P allele may be a marker of osteoporosis in ISA layers.