联合应用神经保护剂抑制大鼠局灶性脑缺血后的神经元凋亡

Combination of neuroprotective agents inhibited neuron apoptosis in focal ischemic area of rat with MCAO

目的探讨联合应用神经保护剂能否较单一用药更好地达到保护脑的作用。方法采用线栓法制作大鼠大脑中动脉闭塞模型(MCAO),分为对照组、1,6-二磷酸果糖(FDP)组、MK-801组、N-乙酰半胱氨酸(NAC)组、联合治疗组和假手术组。应用TUNEL染色检测神经元凋亡,免疫组化及W estern b lot检测caspase-3蛋白表达。结果缺血后6 h和24 h,联合治疗组神经元凋亡和caspase-3蛋白表达均明显少于各单一用药组(P<0.05)。结论联合应用神经保护剂对大鼠局灶性脑缺血的保护作用明显增强。

Objective To investigate the protective effect of therapy with different combined neuroprotective agents. Methods Using suture occlusion technique, the right middle cerebral artery in rats was occluded. All animals were randomly assigned before surgery to following groups： control group, FDP-treated group, MK-801-treated group, NAC-treated group, combination-treated group and sham operation group. The apoptosis of neurons was detected with TUNEL stained. The expression of caspase-3 was detected with immunohistochemistry and Westemblot. Results After 6 and 24 h of cerebral ischemia, beth apoptosis of neurons and the expression of caspase-3 in the combinationtreated group were significantly decreased than those in single agent group （ P 〈 0. 05 ）. Conclusion Combined use of neuroprotective agents may be more effective than single agent.