RP-HPLC法同时测定黄连中四种原小檗碱型生物碱的含量

Simultaneous Determination of Four Berberine-type Alkaloids in Coptis Chinensis Franch Using RP-HPLC

目的：建立用RP-HPLC技术同时测定黄连中小檗碱、巴马亭、黄连碱及药根碱等4种原小檗碱型生物碱含量的方法。方法：采用高效液相色谱法。测定黄连药材中上述4种原小檗型生物碱的含量。结果：4种原小檗碱型生物碱的平均含量分别为：5．74%(RSD=1．7%)(n=9),1．33%(RSD=1．3%)(n=9),3．67%(RSD=2．8%)(n=9),0．95%(RSD=5．2%)(n=9)。结论：该方法可用于同时测定黄连中4种原小檗碱型生物碱的含量,且简便、准确、可靠、快速,并具重现性。

This study is to establish a quantitative method for analyzing the concentration of four berberine - type alkaloids in Coptis chinensis Franch in a simultaneous manner, using RP - HPLC. The RP - HPLC process, made up of Hypersil - 18 Column （250mm × 4.6mm,5μm） , a mixed mobile phase of acetonitrile- Water（48：52, KH2PO4 3.4g, SDS 1.7g every IL）, a flow velocity of 1.0 mL ·min^-1, and a detection wavelength of 345nm, is employed to simultaneously determine the concentration of four Berberine - type Alkaloids in Coprtis chinensis Franch. The study has produced the following results ：linear calibration curves for Berberine, Palmaune, Coptisine, and Jatrorrhizine in a respective range of 0. 024 -0.096mg·mL^-1 and r =0. 9994; 0. 01155 -0. 03005mg ·mL^-1 and r =0. 9994; 0. 00298 -0. 02679mg·mL^-1 and r =0.9992; and 0.0032 -0.0096mg ·mL^-1 and r =0. 9992. The average recovery rates are registered respectively at 102.3% and RSD=3.0%（n=3）; 101.5% andRSD=1.8%（n=3）; 103.7% and RSD=3.6% （n=3）; and 113.3% and RSD = 7.0% （ n = 3 ）. The average concentration of four berberine - type alkaloids in Coptis chinensis Franch is measured at 5.74%（RSD=1.7%）（n=9）;1.33%（RSD=1.3%）（n=9）; 3.67%（RSD=2.8%）（n= 9） ; and 0.95% （RSD = 5.2% ） （n = 9）respectively. It is concluded that the method is simple, accurate, reliable, quick and reproducible, it can be used for quantitative analysis of four berberine - type alkaloids in Coptis chinensis Franch in a simultaneous manner.