慢性酒精中毒大鼠学习记忆及内侧隔核胆碱能神经元的变化

Changes of Learning Memory and of Cholinergic Neurons of Medial Septum Induced by Chronic Alcoholism in Rats

目的研究酒精致大鼠学习记忆障碍与基底前脑内侧隔核胆碱能神经元变化的关系。方法将24只雄性Wister大鼠随机均分成对照组和酒精中毒组,酒精中毒组大鼠隔日胃内注入含酒精(2.5g/kg)的蒸馏水2ml共90d,对照组则注入不含酒精的蒸馏水2ml。然后采用Morris水迷宫和免疫组织化学技术,分析大鼠基底前脑内侧隔核胆碱能神经元、海马星形胶质细胞数的变化和学习记忆能力的关系。结果酒精中毒组大鼠Morris水迷宫潜伏期明显延长,基底前脑内侧隔核胆碱能神经元数目明显减少,海马星形胶质细胞数目反应性增生。结论酒精致大鼠学习记忆能力障碍与基底前脑内侧隔核胆碱能神经元减少有关。

Objective To investigate the effect of chronic alcoholism on the learnig memory as well as on the Chollnergic Neurons of medial septum of adult rats, Methods Healthy adult Wistar rats （n=24） were randomly divided into the following groups： normal control group, chronic alcoholism group（ alcohol was given （2.5g/kg） intragastrically by garages once every other day for 90 days）. Immunohistochemical analyses were made on choline acetytransferase （CHAT）and glial fibrillary acidic protein （GFAP）. The abilities of learning and memory were detected in Morris water maze. Results The number of choline aeetytransferase immunoreactive （CHAT--IR） cells in medial septum and the abilities of learning memory were significantly decreased in chronic alcoholism group （P 〈 0. 01）. Conclusions Alcohol can cause malfunction on learning memory,which is related to the decrease of Cholinergic Neurons of basal forebrain.