杏和李树啤酒花矮化类病毒的检测与序列分析

Detection and Sequence Analysis of Hop stunt viroid in Apricot and Plum

从无明显症状表现的24个杏样品,37个李样品的1年生枝条表皮中抽提低分子RNA进行DIG-cDNA斑点杂交、RT-PCR、正反向聚丙烯酰胺凝胶电泳(Return-PAGE)和生物学检测。结果表明:杏有3个样品,李有6个样品带有啤酒花矮化类病毒(Hop stunt viroid,HSVd)。从这9个样品中选a5、a17、p6、p16进行克隆和测序分析。所得序列与GenBank中已报道的HSVd序列同源性达99.0%～99.7%,且序列变异与地域、寄主等无明显相关性,说明HSVd的序列是比较保守的。

Low molecular mass RNAs extracted from bark of one year old branch of 24 apricot samples, 37 plum samples were used for DIG-cDNA Dot-blot hybridization, RT-PCR, Return-PAGE and biological indexing. There were no clear symptoms when these samples were collected. The result showed that 3 of 24 apricot samples, 6 of 37 plum samples were infected with Hop stunt viroid （HSVd）. aS, a17, p6, p16 selected from the 9 samples were used for cloning and sequencing analysis. HSVd sequence variants obtained were compared with HSVd reported in GenBank and showed 99. 0% - 99. 7% identity. There was no clear relativity among sequence variants, region and host. These results suggest that sequence of HSVd is relatively conserved.