“双固一通”针法对糖尿病大鼠的治疗作用和对T细胞亚群的影响

Effect of Acupuncture Therapy of Shuangguyitong on T cell subtypes of STZ-induced Diabetic Rats

目的:观测“双固一通”针法对糖尿病大鼠的治疗作用和对T细胞亚群的影响。方法:180—220gW istar大鼠73只,13只为正常对照(A组),另60只行链脲左菌素腹腔注射造模,造模成功的大鼠随机分为模型组(B组)、“胰俞”针刺治疗组(C组)、“双固一通”针刺治疗组(D组)和常规针刺治疗组(E组),治疗2个疗程后,用血糖仪检测血糖,放射免疫分析法检测血清胰岛素,运用流式细胞仪检测全血T细胞亚群(CD4+/CD3+、CD8+/CD3+)。结果:C组对糖尿病大鼠血糖、CD4+T和CD8+T细胞比例无显著性影响(P>0.05);D组和E组均明显降低糖尿病大鼠的血糖水平(P<0.05),对降低的胰岛素水平的影响无统计学意义(P>0.05);D组和E组均可使糖尿病大鼠降低的CD4+T细胞比例有不同程度的升高,使升高的CD8+T细胞比例有不同程度的降低,使CD4+T/CD8+T细胞比值不同程度趋于正常水平,组间比较,以D组对T细胞亚群的调节作用最好。结论:“双固一通”针法降糖作用确切,其作用可能不是通过升高血浆胰岛素水平实现的;“双固一通”针法对糖尿病大鼠的免疫功能有明显的调节作用,这可能是其对神经内分泌网络进行调节的重要环节。

Objective ：To investigate the effect of shuangguyitong （ tonifying congenital and acquired essence, eliminating patho- gen） acupuncture therapy on T cell subtypes of streptosotocin （STZ）-induced diabetic rats. Methods： Among seventy-three 180- 220g Wistar rats, thirteen were assigned as normal control group（group A） and sixty were induced diabetes by injecting STZ into celiac cavity. The diabetic rats were randomly divided into model group （group B） , shuangyuyitong treatment group（ group C）, shuangyuyitong acupuncture group（ group D） and common acupuncture group（group E）. After two courses of treatment, plasma in- sulin was tested by radioimmunoassay （RIA） and T cell subtypes （ CD4 ^＋/CD3 ^＋ , CD8^＋/CD3^＋ ） were tested with flow cytometry. The blood glucose was measured by sugar equipment. Results： The high blood glucose of the diabetic rats were lowered significantly in group D and E（ P 〈0.05）. There was no significant difference between the contents of insulin before and after the treatment in group D and E（ P 〉 0.05）. The ratio of CD4 ^＋ Tcell in model group was less than that of normal group, that of CD8^＋ Tcell was higher and the ratio of CD4^＋ and CD8^＋ T cell was lower than that of the normal group. The regulative effect of group D was the best among the treatment groups. Conclusion ： shuangyuyitong acupuncture therapy has reliable effect of down-regulating blood sugar on DM rats. The therapy can adjust the immunity function of DM rats significantly, which might be an important tache to regulate the neuroendocrinoimmune network.