摘要
目的:观察梓醇对氧糖剥夺(OGD)诱导PC12细胞凋亡的保护作用。方法:采用Hoechst 33258 DNA染色法,四甲基偶氮唑盐(MTT)检测细胞活性;化学比色法测定乳酸脱氢酶(LDH)的释放量,用流式细胞技术检测细胞凋亡比例以及P53和Bcl-2蛋白。结果:OGD可导致PC12细胞活力明显下降,LDH释放量增加、P53蛋白表达上升,Bcl-2蛋白表达下降。梓醇可明显改善细胞形态结构,显著降低LDH释放量、降低P53蛋白的表达,提高Bcl-2蛋白的表达,降低细胞凋亡率。结论:梓醇通过调节细胞凋亡相关基因的表达而抑制细胞凋亡。
Objective： To investigate the effect of catalpol in apoptosis in PC12 cells induced by oxygen-glucose deprivation （OGD）. Methods： The morphological changes were observed by Hoechst 33258 staining assay; the cell viability was analyzed by MTT; the apoptosis rate, the protein of P53 and Bd-2 were measured by flow cytometry analysis （FCM）. Results： OGD could result in the decrease of cell viability, the increase of release of LDH, the dropping of Bcl-2 protein expression of PC12 cell, the rising of P53 protein expression of PC12 cell. Catalpol could improve the morphology of cells. It could increase bcl-2 protein expression, decrease P53 protein expression and the release of LDH, and reduce apoptotic rate. Conclusion： Catalpol could inhibit PC12 cells apoptosis by regulating related genes of apoptosis.
出处
《现代生物医学进展》
CAS
2006年第12期31-33,共3页
Progress in Modern Biomedicine
