RP-HPLC测定人参各部位人参皂苷compound K的含量

RP-HPLC determination of ginsenoside compound K in various part of Panax ginseng

目的:考察人参皂苷 compound K 是否存在于人参植物体中并测定其含量。方法:采用氯仿-甲醇(15∶1)超声提取,并用 Zorbax SB C_(18)色谱柱(4.6 mm×150 mm,5μm),以乙腈和水作为流动相进行二元梯度洗脱,流速为0.8 mL·min^(-1),检测波长为203 nm。结果:人参花和人参果中人参皂苷 compound K 的含量分别为0.02%和0.04%。人参皂苷 compound K 在0.523～10.45μg范围内呈良好的线性关系(r=0.9996),平均回收率为97.55%。在人参茎叶、鲜人参主根、红参芦头、红参主根、红参须根中未检出人参皂苷 compound K。结论:在人参植物体中存在人参皂苷 compound K。本方法简便、灵敏和准确,可作为人参皂苷 compound K 的有效分析方法。

Objective：To investigate whether Panax ginseng contains ginsenoside compound K and to quantitate ginsenoside compound K. Method：The samples were extracted with chloroform and methanol（ 15：1 ）. The content of ginsenoside compound K in various part of Panax ginseng was detected by RP - HPLC using Zorbax SB C18 column（4. 6 mm × 150 mm,5μm）. The mobile phase consisted of acetonitrile -water with gradient elution, at a flow rate of 0. 8 mL · min^-1. The detection wavelength was 203 nm. Results：The contents of ginsenoside compound K in the flower and the fruit were 0. 02% and 0. 04% , respectively. Ginsenoside compound K shows good linearity（ r = 0. 9996 ）in the range of 0.523 - 10.45 μg. The average recovery was 97.55%. Ginsenoside compound K was not found in the stem and leaves of ginseng, main root of fresh ginseng, the basal part of stem, fibrous root and main root of red ginseng. Conclusion： Ginsenoside compound K exists in Panax ginseng. This method is simple, sensitive and accurate, and it can be used for the assay of ginsenoside compound K content in various part of Panax ginseng.