大鼠电刺激诱发心室颤动和机械性心肺复苏模型的建立

The Establishment of Novel Rat Model of Electrical Induced Ventricular Fibrillation and Mechanical Cardiopulmonary Resuscitation

目的:建立大鼠交流电电激诱发心室颤动和机械性心肺复苏模型,并探讨其有效性和安全性。方法:选用Sprague-Dawley雄性大鼠20只,应用自主开发研究的心跳骤停和复苏机械装置,持续交流电经右心室内膜致颤。在8min心室颤动后,开始给予6min的心肺复苏,包括200次/min的机械胸外按压和100次/min的同步机械通气。随后双向波经胸体外除颤。结果:14只大鼠复苏成功,自主循环恢复率为70%。电刺激后所有大鼠立刻出现心室颤动,3min的电刺激停止后动物持续表现为室颤而没有自发转复心律现象。心肺复苏期间恢复自主循环组其冠状动脉灌注压恒定在24mmHg左右,显著高于未能恢复自主循环组。大部分大鼠在6min复苏后电击除颤有效恢复自主循环。实验结束后常规解剖未发现器官机械性创伤。结论:本大鼠交流电诱发心室颤动和心肺复苏模型,有效性和安全性高,可最大限度减少实验的误差,具有一定的推广应用前景。

Objective. To establish a novel rat model of electrical induced ventricular fibrillation （VF） and mechanical chest compression and ventilation during cardiopulmonary resuscitation （CPR）, and investigate the efficacy and safety of this rat model. Method： VF was induced and maintained by an alternating current delivered to the right ventricular endocardium in 20 Sprague-Dawley rats. CPR, including mechanical ventilation （100/min） and preconilal compression （200/min） with a pneumatic pistion device was initiated after 8 rain of untreated VF. Defibrillation was attempted after 6 win of CPR. Result： Forteen animals were successfully resuscitated. The rate of restoration of spontaneous circulation （ROSC） was 70%. VF immediately occurred after initiating electrical stimulation. No spontaneous cardiovertion was observed during VF period. The coronary perfusion pressure （CPP） was maintained at -24 mmHg in animals with ROSC, which was significantly greater than those without ROSC. The majority of animals were successfully defibrillated 6 min after resuscitation. No gross abnormalities were observed at autopsy in any of the animals at the end of experiment. Conclusion： This novel rat model of electrical induced VF and mechanical chest compression and ventilation is characterized by satisfied efficacy and safety, which can minimize the experimental bias. Therefore it can standardize the rat model of CPR and provide a good tool for basic research of CPR.