不同转移特性人肺癌裸鼠移植瘤模型的建立及复制

Establishment and replication of human lung carcinoma metastasis models in nude mice

以人肺巨细胞癌细胞系ＰＬＡ－８０１的两个细胞克隆Ｃ株及Ｄ株，建立了ＰＬＡ－８０１Ｃ及ＰＬＡ－８０１Ｄ裸鼠皮下移植瘤模型，以人肺腺癌裸鼠移植瘤培养细胞Ａｎｉｐ９７３，复制了Ａｎｉｐ９７３裸鼠皮下移植瘤及腹水瘤模型，对其生物学特性进行了研究。这４株移植瘤模型的淋巴结及肺转移率由低到高依次为ＰＬＡ－８０１Ｃ皮下瘤（０％，０％）、Ａｎｉｐ９７３皮下瘤（８．６％，４．３％）、Ａｎｉｐ９７３腹水瘤（１００％，１２．０％）、ＰＬＡ－８０１Ｄ皮下瘤（１００％，３１．６％）。

Two strains of human lung carcinoma metastasis models were established and two strains of human lung adenocarcinoma were replicated in nude mice in the following procedure: (1) The nude mice were inoculated subcutaneously with cellular colonal strains C and D isolated from a human lung giant carcinoma cell line PLA 801 to establish two models of subcutaneously transplanted carcinoma PLA 801C and PLA 801D; (2) Two models were replicated respectively through subcutaneous and intraperitoneal inoculation of human lung adenocarcinoma cell Anip973 cultivated from its transplanted xenograft. It was found that the biological characteristics of the four strains were stable and their metastatic rates to lymph node and lung were in order from low to high among subcutaneous PLA 801C (0%, 0%), subcutaneous Anip973 (8.6%, 4.3%), ascitic Anip973 (100%, 12.0%) and subcutaneous PLA 801D (100%, 31.6%). The establishment of transplanted models of human lung carcinoma from the same origin and with various metastatic potentials in nude mice is of great significance for the comparative study of mechanisms of lung carcinoma metastasis.