大肠癌组织bFGF,PKB,Cyclin A的表达及其与临床的相关性

Expression of basic flbroblast growth factor,protein kinase B,Cyclin A and their clincial significances in colorectal cancer

目的：研究大肠癌组织中碱性成纤维细胞生长因子（bFGF），蛋白激酶B（PKB）及细胞周期蛋白A（Cyclin A）mRNA的表达水平及与临床病理的关系．方法：采用TRIzol法分别提取60例大肠癌组织，癌旁组织和10例正常组织的RNA，应用RT-PCR方法检测bFGF,PKB。Cyclin A mRNA的表达水平，PCR产物经凝胶成像及分析系统扫描后，以β-actin为参照标化bFGF，PKB，Cyclin A的含量．通过统计学分析其与临床病理关系．结果：bFGF,PKB，Cyclin A在大肠癌组织中的表达与癌旁组织相比有显著差异（bFGF：60％vs10％：PKB：55％vs35％：Cyclin A：70％vs5％：allP〈0．05），其表达水平与大肠癌Dukes分期有关（bFGF：χ^2=4．434，P〈0．05；PKB：χ^2=13．549，P〈0．01：Cyclin A：χ^2=21．210，P〈0．01）．PKB在低分化肿瘤中的表达阳性率高于中高分化肿瘤（14／14vs29／46，P〈0．05）．CyclinA的表达在高、中分化肿瘤中明显高于低分化肿瘤（37/46vs3/14,P〈0.05）结论：bFGF,PKB,Cyclin A的mRNA水平与大肠癌的发生,发展相关.

AIM： To investigate the expression of basic fibroblast growth factor （bFGF）, protein kinase B （PKB）, and Cyclin A in colorectal cancer and their correlations with the clinical pathology. METHODS： After RNA was extracted by TRIzol method from 60 cases of colorectal cancer, their adjacent tissues and 10 cases of normal colorectal tissues, reverse transcriptase polymerase chain reaction （RT-PCR） was used to detect the mRNA expression of bFGF, PKB, Cyclin A. The correlation between the results and clinical pathology was analyzed. RESULTS： Compared with those in the canceradjacent tissues, the expression of bFGF, PKB, Cyclin A mRNA were significantly up-regulated in colorectal cancer tissues （bFGF： 60% vs 10%; PKB： 55% vs 35%; Cyclin A： 70% vs 5%; all P 〈0.05）, which were significantly correlated with Dukes stages （bFGF： χ^2 = 4.434, P 〈 0.05; PKB： χ^2 = 13.549, P 〈 0.01; Cyclin A： χ^2 = 21.210, P 〈 0.01）. The level of PKB expression was markedly higher in lowly-differentiated tumor than that in highly and moderately-differentiated one （14/14 vs 29/46, P 〈 0.05）, but Cyclin A expression showed reverse results （3/14 vs 37/46, P 〈 0.05）. CONCLUSION： The expression of bFGF, PKB, Cyclin A mRNA may play important roles in the carcinogenesis and development of colorectal cancer.