δ阿片受体激动剂对失血性休克大鼠血流动力学的影响

Effects of δ opioid receptor agonist D-Aia^2-D-Leu^5-enkephalin on hemodynamics in rats with hemorrhagic shock

目的 探讨δ阿片受体激动剂D-Ala^2-D-Leu^5-enkephalin（DADLE）对失血性休克大鼠血压和心功能的影响。方法 36只健康SD大鼠，随机分成假手术组、休克对照组和DADLE组（1mg／kg组和5mg／ks组），休克对照组和DADLE组大鼠放血使MAP降至40mmHg，维持低血比状态（MAP=40mmHg）60min后，用生理盐水或DADLE复苏，观察复苏后3h内动脉血压（SAP、DAP和MAP）和心功能指标（LVSP、±dp／dtmax）的变化。结果 休克对照组复苏后各时点动脉血压和心功能指标呈逐渐下降趋势。DADLE组复苏后5min动脉血压和心功能指标开始升高，30min达高峰，复苏后同一时点各项指标均显著高于对照组（P〈0．01），其中1mg／kg组和5mg／kg组没有显著差异。结论 DADLE能改善失血性休克大鼠的血流动力学指标。

Objective To investigate effects of D-Ala^2-D-Leu^5-enkephaiin （DADLE） on hernodynomies in rats with hemorrhagic shock. Method Thirty-six healthy male SD rots were randomly divided into sham group, control group and DADLE group （ 1 mg/kg group and 5 mg/kg group）. The animals in control and DADLE groups were exsanguinated till MAP decreased to 40 mmHg. When MAP maintained 40 mmHg for 60 minutes, rots were resuscitated with normal saline or DADLE. Arterial blood pressure （SAP, DAP, MAP）, cardiac function （LVSP and ＋ dp/dtmax ） were recorded after resuscitation for 3 hours. Results After hemorrhagic shock and resuscitation, arterial blood pressure and cardiac function gradually decreased in the control group. While arterial blood pressure and cardiac function began to increase at 5 mimutes after resuscitation and reached peak at 30 minutes in DADLE （ l mg/kg or 5 mg/kg） group, and the data were significantly different compared with the control group （P 〈 0.01） . There was no significant difference between 1 mg/kg and 5 mg/kg DADLE groups. Conclusions DADLE could improve the hemodynanfics during the course of hemorrhagic shock.