环境物质稀土多元复合肥对玉米根尖细胞DNA的损伤

The Damage of Rare Earth Multi-element Compound Fertilizer of Environment Substance on Root-tip Cell DNA of Zea Mays L

有关对稀土多元复合肥(RE-MECF)的卫生毒理研究较多,但对其遗传毒理研究报道较少。在此,采用了单细胞凝胶电泳技术,探知RE-MECF对玉米细胞DNA是否会产生损伤,以期引起注意。用不同梯度浓度的RE-MECF对农作物玉米进行染毒6h后,将玉米根尖细胞制备成原生质体,再进行单细胞凝胶电泳,确定对DNA损伤并探讨其剂量-效应关系。结果显示,质量浓度为10mg·L^(-1)剂量组与阴性组比较没有显著性差异;质量浓度在10~2～10~3mg·L^(-1)剂量范围内彗星细胞的头长随着剂量的增加而缩短,而尾长随着剂量的递增而增长。与阴性组比较存在显著性差异,DNA拖尾率、损伤率随着剂量的增加相应升高;质量浓度在10~4mg·L^(-1)剂量组彗星的头长和尾长与阴性组相比皆无显著性差异。实验结果表明:RE-MECF对玉米根尖细胞DNA具有一定的损伤作用。

At the present time, the reports on the healthy toxicology are more than that on the genotoxie toxicology of the rare earth multi-element compound fertilizer. This research used the single cell gel electrophoresis （SCGE） technology to study the DNA damage in root-tip cells of Zea mays L. The root tips of maize were treated with different concentrations of RE-MECF for 6 h , and processed them into pretoplast ,then observed the morphology and accounted mortality of cells , finally we performed single cell gel electrophoreais （SCGE） to determine DNA damage . There was no significant difference between 10 mg·L^-1 group and the control group . In dosage from 102 mg·L^-1 to 103 mg·L^-1, the average head length diminished gradually with the rise of dosage compared with the control （P 〈 0.01） , while the average tail length increased significantly on the contrary, and the ratio of damaged DNA also increased with the rise of dosage. However, there was no remarkable difference compared with the negative of the group of 104 mg·L^-1 . It suggests that RE-MECF may damage DNA in root -tip cells of maize in some degree.