慢性饮酒对大鼠急性肺损伤的影响

Influence of chronic alcohol intake on acute lung injury in rats

目的研究慢性饮酒对大鼠注射油酸-脂多糖致急性肺损伤(ALI)的影响,以及损伤后肺组织氧化/抗氧化平衡的改变。方法32只SD大鼠随机予以慢性饮酒或饮水;6周后随机注射油酸-脂多糖致ALI(损伤组),或注射生理盐水作为对照(对照组)。共分为4组饮酒损伤组、饮水损伤组、饮酒对照组和饮水对照组,每组8只动物。检测其PaO2,肺组织中还原型谷胱苷肽(GSH)和丙二醛(MDA)水平,以及肺组织干质量/湿质量比值(W/D)的变化。结果与相应对照组比较,饮酒损伤组和饮水损伤组PaO2明显下降,W/D值明显增加,GSH水平降低,MDA水平升高(P均<0.05)。饮酒损伤组与饮水损伤组比较,除W/D值变化无显著差异外,其余指标下降均更显著(P均<0.05)。结论慢性饮酒可增加创伤后感染时肺损伤的严重程度,与肺组织氧化/抗氧化失衡有关。

Objective To investigate the influence of chronic alcohol ingestion on the severity of acute lung injury （ALI） induced by oleic acid and lipopolysaccharide （LPS）. Methods Thirtyotwo SD rats were randomly administrated with alcohol or water for 6 weeks,then instilled with oleic acid and LPS to induce ALl or with normal saline as control. Thus the rats were randomly divided into two injury groups [ ethanol group and water group] and two control groups [ ethanol group and water group] （ n = 8 in each group）. PaO2,Wet to dry lung weight ratio （W/D）, levels of γ-glutamylcysteinylglycine （GSH） and malonaldehyde （MDA） in the lung tissue were measured. Results Compared to corresponding control groups, the PaO2 and GSH significantly decreased, and the lung W/D and MDA level were significantly increased in the injury groups （ all P 〈 0.05）. In the injury groups, the changes of above parameters were more significant in the alcohol group than those in the water group （all P 〈 0.05）, except the lung W/D with no significant difference. Conclusion Chronic ethanol ingestion was relevalent to oxidation/antioxidation imbalance and more severe lung injury in rats with severe septic after trauma, which suggests that chronic alcohol abuse could increase the severity of acute lung injury.