疏肝解郁中药对肝郁大鼠胃黏膜结构与功能的影响

Influence of the coursing liver-qi and resolving depression Chinese medicines on the structure and function of the gastric mucous membrane in rats with liver-qi stagnation

[目的]通过动物实验应用疏肝解郁中药以方测证,以期揭示肝郁及胃的病理生理基础。[方法]绑带束缚14 d后致大鼠肝郁。观察比较正常组、肝郁组、复健组大鼠胃黏膜病理组织学变化,检测大鼠胃液中游离黏液量、前列腺素E2(PGE2)及胃黏膜组织匀浆液中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)水平。[结果]肝郁组大鼠胃黏膜出现炎性改变,余2组无显著变化;肝郁组大鼠胃液中游离黏液量、PGE2水平低于其余2组,且胃黏膜组织匀浆液中的SOD、NO水平均低于其余2组,MDA高于其余2组。[结论]疏肝解郁中药可有效预防肝郁及胃病理变化,增强胃黏膜防御保护功能。

[Objective] To reveal the pathological and physiological functions of stagnation of live-qi referring to stomach by applying the coursing liver-qi and resolving depression Chinese medicines, and to measure the syndrome by the prescription, [Methods]After 14 days of constraint by band, rat models of liver-qi stagnation were established. The pathological changes of the gastric mucous membrane in the normal group were observed, the model group and the recovery group. The concentration of free mucus and PGE-2 was detected in gastric juice. The level of SOD, MDA,NO in gastric mucous membrane homogenate was assayed. [Result]1 The gastric mucous membrane of the model group appeared inflammatory changes, normal group and model group had no significant changes;The free mucus and PGE-2 of the model group was lower than that of the other two groups;SOD and NO level in gastric mucous membrane homogenate of the model group was lower than that of the other two groups, but MDA of the model group was higher. [Conclusion]The coursing liver-qi and resolving depression Chinese medicines can prevent the pathological change of the stagnation of liver-qi referring to the stomach by strengthening the defensive and protective functions of the gastric mucous membrane.