金黄地鼠高血脂模型的建立

Establishment of Golden Hamster Model of Hyperlipidemia

目的建立高血脂金黄地鼠动物模型,研究红葡萄酒预防高血脂的作用。方法用含有2%胆固醇的高脂饲料喂养金黄地鼠,设正常对照组和模型组,饲养15 d。期间观察金黄地鼠对高脂饲料的耐受性,试验结束时,测量血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、极低密度脂蛋白胆固醇(VLDL-C)值,并对肝脏进行光镜和电镜观察。结果金黄地鼠对高脂饲料具有较强的耐受性。经过15 d之后,模型组TC、TG、HDL-C、LDL-C、VLDL-C极显著升高(P<0.01),分别为对照组的9.06、4.19、2.43、6.21、18.88倍,而HDL-C/(LDL-C+VLDL-C)显著降低(P<0.05),光镜和电镜观察表明模型组动物的肝脏出现脂肪肝样改变。结论本造型方法时间短,取血方便,血量充足,是一个较好的高血脂模型建立方法。

Objective To establish a golden hamster model of hyperlipidemia. Methods 20 golden hamsters were randomly divided into control and experimental groups, 10 per each group. The hamsters of experimental group were treated with high cholesterol diet for 15 days. During the experiment, the gross health status was observed. Finally, the contents of serum total cholesterol （T C）, triglyceride （TG）, high-density lipeprotein-cholesterol （HDL-C）, low-density lipeprotein-cholesterol （LDL-C） and very low-density lipeprotein-cholesterol （VLDL-C） were assayed, and pathological changes of the liver were examined by light and electron microscopy. Results The golden hamsters showed to be well toleranble to high cholesterol diet. After 15 days, the contents of serum TC, TG, HDL-C, LDL-C and VLDL-C were 9.06,4.19,2.43,6.21,18.88 fold-increased, respectively （P 〈 0.01 ）, but the ratio of HDL-C/（ LDL-C ＋ VLDL-C） decreased significantly compared with that in the normal group （P 〈 0.05）. The pathological examination exhibited liver structural alterations. Conclusion it is a quite rapid and reliable way to establish hyperlipidemia model of golden hamster, which can continuously provide enough plasma for hyperlipidemia research.