咪喹莫特对人皮肤成纤维细胞活性和凋亡的影响

Imiquimod effects on the apoptosis and activity of fibroblasts in vitro

目的:观察咪喹莫特(imiquimod)溶液对人真皮成纤维细胞(FB)细胞毒性、细胞增殖及细胞凋亡率的影响,探讨其治疗瘢痕疙瘩的可能机制。方法:分离培养人真皮成纤维细胞,用四甲基偶氮唑蓝(MTT)法检测不同浓度咪喹莫特溶液对成纤维细胞毒性及细胞增殖的影响;用流式细胞技术检测咪喹莫特对成纤维细胞凋亡率的影响。结果:不同浓度咪喹莫特加入成纤维细胞培养体系孵育24h后,成纤维细胞形态均有不同程度受损,且细胞增殖活性下降,尤以20mg/L浓度时细胞活性下降明显;各浓度咪喹莫特处理组细胞凋亡率均高于对照组(P<0.05),但在不同时间点检测的细胞凋亡率差异无统计学意义(P>0.05)。结论:咪喹莫特可以降低成纤维细胞增殖活性并增加细胞凋亡率,这些可能是其促进皮肤瘢痕组织消退的相关机制。

Objective： To observe the effect of imiquimod on the cytotoxicity, the cell proliferation and the apoptosis rate in human skin fibroblasts. Methods： The cytotoxicity and proliferation were detected by MTr. The apoptosis rate was detected by flow cytometry （FCM）. Results： In the imiquimod （20 μg/mL） treated groups the fibroblasts were significantly damaged and the proliferation activity was decreased. The cellular apoptosis rates in different concentration of imiquimod-treated groups were statistically higher than those in control group （P 〈 0.05）. There was no statistical difference in the rates of apoptosis at the time point experiment （P 〉 0.05）. Conclusion： The results indicate that imiquimod could decrease the cellular proliferation activity and increase the apoptosis rate of human skin fibroblasts, which might become one of the possible mechanisms for effectively treating cicatricial tissue.