高效液相色谱法测定皮炎露中苯酚的含量

Determination of phenol in dermatitis Liniment By HPLC

建立高效液相色谱法测定皮炎露中苯酚含量。方法：以μBondapak C18为固定相，甲醇-水（75：25）为流动相，检测波长为240nm。结果：苯酚浓度在1000～3000mg／L范围内线性关系良好。相关系数为r=0．9997；平均回收率100．56％（n=9）；重现性良好（n=6），RSD1．42％。结论：本方法简单、快速，结果准确，适于该制剂的含量测定。

OBJECTIVE To estabLish a method for determination of phenol in dermatitis Liniment by HPLC. METHODS： TheBondapak C18 was used. The mobile phase was methanol -water（75： 25） ,the detection wave length was set at 240 nm. RESULTS In the scope of 1 000 - 3 000 mg/L for phenol had good Linear relationship with their peak area（ r = 0.999 7 ）. The average recovery was 100.56% （n =9）. The precision of the method was good, RSD = 1.42%. CONCLUSION： The method is simple,rapidly and accurate. It is suitable for determination of the preparation.