肉苁蓉总苷前体脂质体的研制

The Preparation of the Cistanosides proliposome

目的探讨肉苁蓉总苷前体脂质体的各项理化性质及水溶性天然药物提取物制成前体脂质体的可行性。方法采用新型前体脂质体制备技术,将水溶性药物肉苁蓉总苷制成肉苁蓉总苷前体脂质体;采用透射电镜观察形成的脂质体形态;采用粒径测定仪测定脂质体粒径;采用葡聚糖凝胶柱洗脱法测定脂质体包封率。结果电镜下脂质体呈球形,外层隐约可见有多层。脂质体粒径小,粒度分布均匀,脂质体稀释倍数越大,粒径越小,ξ电位在-21.05mV,包封率值为(24.98±0.76)%。结论新型前体脂质体制备技术可将水溶性天然药物提取物肉苁蓉总苷包封在脂质体内,初步说明前体脂质体制备具有可行性。

Objective： To make the cistanosides proliposome with a new method and study the properties of the proliposome. Methods： The cistanche glycosides was made into proliposome with a new method, and then its shape was observed by the transmission electron microscope, and the particle sizes were determined, finally the trap efficiency was studied by the sephadex column layer analysis. Results： The particles of the preparation were round with small particle sizes, and uniform size distribution. When the diluion times became larger, the particle sizes were smaller. Furthermore, the electronic potenial was -21.05 mV and the trap efficiency was （24.98±0.76） %. Conclusions： The new method can trap the cistanosides with the water-solubility, and it is feasible.