红茶菌调节小鼠免疫功能的实验研究

The Effect of Black Tea Fungus on Immunological Function of Mice

目的探讨红茶菌对小鼠免疫功能的调节作用。方法Balb／c小鼠30只，体重18～22g，随机分为两组，用红茶菌液给小鼠灌胃30d后处死，以MTT测定NK细胞的杀伤活性；以腹腔巨噬细胞吞噬白色念珠菌的方法评价巨噬细胞吞噬功能；以胸腺细胞增殖法和脾细胞增殖法分别测定IL-1和IL-2的生物活性。结果红茶菌能显著提高NK细胞的杀伤活性；巨噬细胞吞噬功能明显增强；红茶菌灌胃组IL-1和IL-2活性分别为：0．549±0．024，0、405±0．018：对照组分别为：0．341±0．021，0．361±0．011，实验组与对照组比较，IL—1，IL-2活性得到显著提高（P〈0．01）。结论红茶菌能显著增强小鼠的免疫功能。

Objective To investigate the regulative effect of black tea fungus on the immunological function of mice. Methods Thirty Balb/c mice（weight 18 -22 g） were randomly divided into two groups. All the mice were sacrificed after they had been given black tea fungus in intragastric administration for thirty days. The killing activity of NK cells were detected by MTT method, and peritoneal macrophage swallowing C. albicans test was used to appraise the phagocytes function. The activities of intedeukin 1 and interleukin 2 were separately detected by mouse thymocyte or splenic cell proliferation test. Results The activities of IL - 1 and IL - 2 of mice were 0.549 ± 0. 024, 0. 405 ± 0. 018 in experimental group respectively ; they were 0. 341 ± 0. 021 and 0. 361 ±0.011 respectively in control group. They were significantly higher in experimental group than those in control group （P 〈 0.01 ）. The killing activity of NK cell and the phagocytes function of the macrophages in vitro were also markedly promoted（ P 〈 0.01 ）, Conclusion The black tea fungus can enhance the immunological function of mice significantly.