西洋参叶20s-原人参二醇组皂苷对大鼠实验性心室重构的影响

Effects of Panax quinquefolium 20s-protopanaxdiol Saponins on Experimental Ventricular Remodeling in Rats

目的研究西洋参叶20s-原人参二醇组皂苷（Panax quinquefolium 20s—protopanaxdiolsaponins，PQDS）对大鼠实验性心室重构的防治作用。方法大鼠结扎左冠状动脉前降支4周造成心肌梗死后心室重构模型，同时应用PQDS进行治疗，给药4周后测定心室重构大鼠的心脏形态学、血流动力学及生物化学等参数。结果ipPQDS25～10t）mg·kg^-1，对心室重构大鼠，能明显升高左心室内压最大上升和下降速率（±dp／dtmax）及其校正值[±dp／dtmax）／LVSP]，降低左心室舒张末压（LVEDP），亦能明显降低左心室容积（LVV）、左心室长轴（LVLA）长度、左心室短轴（LVSA）长度、左心室绝对重量（LVAW）、左心室相对重量（LVRW），但对右心室绝对重量（RVAW）、右心室相对重量（RVRW）、心率（HR）、左心室收缩压（LVSP）、平均动脉压（MAP）及体重（BW）均无明显影响。此外，PQDS可明显降低血清脂质过氧化物（LPO）及心肌血管紧张素Ⅱ（AngⅡ）和肾上腺素（E）含量，提高超氧化物歧化酶（SOD）、过氧化氢酶（CAT）及谷胱甘肽过氧化物酶（GSH-Px）活性。结论PQDS能有效防治大鼠心肌梗死后的心室重构。可能与抑制心脏局部AngⅡ生成和抑制交感神经末梢释放CA以及提高心肌的抗氧化能力有关。

OBJECTIVE To study the effects of Panax quinquefolium 20s-protopanaxdiolsaponins （PQDS） extracted from leaves of Panax quinquefolium on experimental ventricular remodeling in rats. METHODS The ventricular remodeling model was induced by myocardial infarction for 4 weeks in rats with left anterior descending coronary occulusion. Mter 4 weeks of treatment with PQDS, hemodynamic changes, biochemical analysis and morphological parameters were determined in ventricular remodeling rats with myocardial infarction. RESULTS Mter being treated by PQDS （ in a dosage of 25 ～ 100 mg · kg^-1 ip for 4 weeks）, the maximum left ventricular pressure rising and dropping rates （±dp/dtmax ） and their left ventricular systolic pressure corrected values（ ±dp/dtmax/LVSP） were increased significantly. The left ventricular end diastolic pressure （LVEDP）, the left ventricular volume（ LVV）, the left ventricular long and short axis（ LVLA and LVSA） , the left ventricular absolute and relative weight （LVAW and LVRW） were reduced significantly, but there were no significant influences on the right ventricular absolute and relative weight （RVAW and RVRW） , the heart rate （HR）, the left ventricular systolic pressure（ LVSP）, mean artery pressure （MAP） and body weight （BW）. In addition, the serum lipid peroxidation （LPO） content, myocardial angiotensin Ⅱ （ AngⅡ ） and epinephrine （E） contents were declined, and the serum superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GSH-Px） activities were increased markedly. CONCLUSION PQDS can efficiently prevent and treat ventricular remodeling in rats with myocardial infarction, which may be related to the inhibition of promoting growth action of Ang Ⅱ and CA, and the elimination of the free radicals in rats with ventricular remodeling induced by myocardial infarction.