柴胡加龙骨牡蛎汤对大鼠心理应激反应的影响

Effect of Chaihu Plus Longgumuli Decoction on Psychological Stress Reaction in Rats

[目的]观察柴胡加龙骨牡蛎汤对急性心理应激大鼠旷场行为和血浆促肾上腺皮质激素的影响。[方法]采用大鼠急性心理应激动物模型,在应激前用两种不同浓度的柴胡加龙骨牡蛎汤胃饲7d,观察心理应激后血浆促肾上腺皮质激素含量的动态变化以及大鼠旷场行为的改变,并与对照组相比较。[结果]与正常对照组比较,应激大鼠在旷场中的穿行格数和直立次数明显增加,血浆促肾上腺皮质激素浓度显著升高,而两种不同浓度的给药,对此反应均有抑制作用。[结论]柴胡加龙骨牡蛎汤对急性心理应激大鼠的心理行为反应和生理反应具有调节作用。

[Objective] The purpose is to determine the effect of Chaihu Plus Longgumuli Decoction （CD） on behaviors and plasma adrenocorticotropic hormone （ACTH） level changed by psychological stress in rats. [Methods] Male Sprague-Dawley rats were randomly allocated to four groups as follows： normal （C）, psychological stress model （P）, CD （12.5g/ kg, ig ） ＋P （DP）, and CD （25g/ kg , ig ）＋P （HP）. One week later, rats were subjected to psychological stress. The behaviors of rats were tested in open field conditions, and the plasma ACTH level was detected by radioimmunoassay. [Results] The crossing scores, the rearing cores and plasma ACTH level increased more significantly in the psychological stress model rats than that in normal group, Rats in both DP and HP groups showed lower crossing and rearing cores and blunted elevation of plasma ACTH after psychological stress compared with rats in psychological stress model. [Conclusion] The hypothalamo-pituitary-adrenocortical （HPA） axis function and anxiety-like behavior could be regulated by CD during psychological stress in rats.