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基质金属蛋白酶与子痫前期发病关系的研究 被引量:11

Differences between the expression of matrix metalloproteinase-2.9 in preeclampsia and normal placental tissues
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摘要 目的探讨基质金属蛋白酶(MMP)2、9在子痫前期患者胎盘绒毛中的表达及其与子痫前期发病的关系。方法采用免疫组化链霉亲和素-生物素-过氧化物酶复合物(SABC)法检测20例子痫前期患者(病例组)和20例正常孕妇(对照组)胎盘绒毛 MMP-2、9的表达,实时荧光定量RT-PCR 检测两组孕妇胎盘绒毛 MMP-2、9 mRNA 的表达水平。因提取后的 mRNA 部分降解,用于RT-PCR 检测的胎盘绒毛病例组13份,对照组10份。采用2^(-△△Ct)相对定量表示 PCR 结果。结果对照组孕妇胎盘绒毛 MMP-2、9的染色强度均高于病例组,两组比较,差异有统计学意义(P<0.05);对照组胎盘绒毛 MMP-2 mRNA 的平均表达水平为7.6±2.8,病例组胎盘绒毛 MMP-2 mRNA 的平均表达水平为5.6±1.5,两组比较,差异有统计学意义(P<0.05)。对照组胎盘绒毛 MMP-9 mRNA 的平均表达水平为2.2±2.6,高于病例组的-0.9±2.0,两组比较,差异也有统计学意义(P<0.05)。对照组胎盘绒毛 MMP-2的平均表达水平高于 MMP-9,两者比较,差异有统计学意义(P<0.05)。结论MMP-2、9表达降低导致的滋养细胞侵袭能力的下降,可能与子痫前期的发病相关。 Objective To study the possible relationships between expression of matrix metalloproteinase (MMP)2,9 and the pathogenesis of preeclampsia in which trophoblast invasion is impaired. Methods MMP-2, 9 expression were detected by immunohistochemistry streptavidin-biotin complex (SABC) method in 20 normal term placentae and 20 preeclampsia placentae, respectively. In addition, mRNAs for MMP-2,9 were analyzed by real time PCR in both groups. Results The intensities of both MMP-2 and MMP-9 immunostaining in precclampsia placentae were significantly declined compared to those of normal term placentae ( P 〈 0. 05 ). By using 2^-△△Ct as a relatively quantitative assay, mRNA expression of MMP-2 was significantly higher in 10 normal placentae than those in 13 preeclampsia placentae (7. 6 +2. 8 vs 5.6 + 1.5 ,P 〈0. 05). Expression of MMP-9 mRNA was also significantly higher in normal placentae than those in preeclampsia placentae (2. 2 ± 2. 6 vs - 0. 9 ± 2. 0, P 〈 0. 05 ). Whereas, mRNA expression of MMP-2 was higher compared to MMP-9 in normal term placentae (P 〈 0. 05). Conclusion The decreased expression of MMP-2 and MMP-9 in preeclampsia placentae may lead to impaired invasion of trophoblast cells, causing abnormal placentation and occurrence of preeclampsia.
出处 《中华妇产科杂志》 CAS CSCD 北大核心 2007年第2期73-75,共3页 Chinese Journal of Obstetrics and Gynecology
基金 四川省卫生厅重点科技项目(02SY029-142)
关键词 先兆子痫 明胶酶A 明胶酶B 胎盘 聚合酶链反应 Pre-eclampsia Gelatinase A Gelatinase B Placenta Polymerase chain reaction
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