摘要
目的:体外分离、培养人乳牙牙髓干细胞。方法:采用酶消化法将人乳牙牙髓分离培养,以获得人乳牙牙髓干细胞。有限稀释法分离纯化,测定细胞克隆形成率,细胞计数法测生长曲线,细胞爬片行HE染色、碱性磷酸酶染色、抗波形蛋白(vimentin)免疫组化染色。结果:通过有限稀释法获得了克隆形成率为11～24个/103,细胞群体倍增时间为39.84h,HE染色细胞形态为梭形、细胞体小、胞核大的干细胞。且碱性磷酸酶染色阳性,抗波形蛋白(vimentin)免疫组化染色阳性。结论:人乳牙牙髓中可分离培养出牙髓干细胞。
Objective： To isolate, culture and identify dental pulp stem cells from human deciduous teeth. Methods： Dental pulp stem cells from deciduous teeth were collected with enzyme digestion, isolated and purified by limited dilution. Examined the cells clone formation rate, measurement of cells growth curve by counting the number of cells, HE and alkaline phophatase （AKP）stain, and immunhistochemistry stain of vimentin. Result： Stem cells were obtained. The cells clone form rate was 11-24 clones/10^3 cells, cells group double time was 39.84 hours. AKP stain and immunhistochemistry stain of vimentin were both positive. Conclusion： Dental pulp stem cells can be collected from deciduous teeth successfully.
出处
《口腔颌面外科杂志》
CAS
2007年第1期25-28,共4页
Journal of Oral and Maxillofacial Surgery
关键词
乳牙
干细胞
分离
细胞培养
deciduous teeth
stem cells
isolate
cell cluture
