丹参对小鼠沙眼衣原体输卵管炎模型影响的实验研究

Effects of Salvia miltiorrhiza on Chlamydia trachomatis mice of salpingitis

目的:探讨丹参在治疗沙眼衣原体慢性输卵管炎和纤维化中的作用。方法:通过阴道内接种建立沙眼衣原体输卵管炎小鼠模型;感染后第3周分不同处理组:只用阿奇霉素治疗组,早期联合丹参治疗组和晚期联合丹参治疗组,于感染第10周,观察输卵管的病变、进行组织病理学观察和胶原增生指数分析。结果:与模型组比较,3组治疗组小鼠出现输卵管阻塞、积水的并发症发生率和胶原增生指数降低有统计学差异(P<0.05)。早期给丹参组与只用阿奇霉素治疗组和晚期给丹参组比较,出现输卵管阻塞、积水的并发症发生率和胶原增生指数降低亦有显著统计学差异(P<0.05)。结论:在用阿奇霉素治疗同时,早期联合丹参治疗,可明显减少沙眼衣原体感染致输卵管阻塞、积水的并发症发生;丹参抑制纤维化可能是其药理机制之一。

Objective： To study the effects of Salvia miltiorrhiza on treatment of Chlamydia trachomatis salpingitis （CTS） and fibrosis. Method： A mouse model for CTS was estahlished in C3H/He by intravaginal inoculation, after 3 weeks mice were randomly divided into 3 groups. Only Azithromyxin was given orally, Azithromyxin and early S. miltiorrhiza given, or ,Azithromyxin and later S. miltiorrhiza given. After 10 weeks, observe the change of oviduct of mice, observe the histopathologic change and analysis collagen histochemical index. Result： 3 Treatment groups induce tubal occlusion and hydrosalpinx decreased and the collagen histochemical index decreased significantly than those of no treatment given （ P 〈 0.05 ）. Early S. miltiorrhiza given group induce tubal occlusion and hydrosalpinx decreased and the collagen histochemical index decreased significantly than only Azithromyxin group or later S. miltiorrhiza given group （P 〈 0. 05）. Conclusion- When we treat CTS genital infection with Azithromyxin, if we can give S. miltiorrhiza treatment as early as possible, it may decrease tubal occlusion and hydrosalpinx, significantly inhibit fibrosis maybe one of its pharmacologic mechanismin.